Expression and regulation of soluble epoxide hydrolase in adipose tissue

Bart M. De Taeye, Christophe Morisseau, Julie Coyle, Joseph W. Covington, Ayala Luria, Jun Yang, Sheila B. Murphy, David B. Friedman, Bruce B. Hammock, Douglas E. Vaughan

Research output: Contribution to journalArticle

60 Citations (Scopus)

Abstract

Obesity is an increasingly important public health issue reaching epidemic proportions. Visceral obesity has been defined as an important element of the metabolic syndrome and expansion of the visceral fat mass has been shown to contribute to the development of insulin resistance and cardiovascular disease. To identify novel contributors to cardiovascular and metabolic abnormalities in obesity, we analyzed the adipose proteome and identified soluble epoxide hydrolase (sEH) in the epididymal fat pad from C57BL/6J mice that received either a regular diet or a western diet. sEH was synthesized in adipocytes and expression levels increased upon differentiation of 3T3-L1 preadipocytes. Although normalized sEH mRNA and protein levels did not differ in the fat pads from mice receiving a regular or a western diet, total adipose sEH activity was higher in the obese mice, even after normalization for body weight. Furthermore, peroxisome proliferator-activated receptor γ (PPARγ) agonists increased the expression of sEH in mature 3T3-L1 adipocytes in vitro and in adipose tissue in vivo. Considering the established role for sEH in inflammation, cardiovascular diseases, and lipid metabolism, and the suggested involvement of sEH in the development of type 2 diabetes, our study has identified adipose sEH as a potential novel therapeutic target that might affect the development of metabolic and cardiovascular abnormalities in obesity.

Original languageEnglish (US)
Pages (from-to)489-498
Number of pages10
JournalObesity
Volume18
Issue number3
DOIs
StatePublished - Mar 2010

Fingerprint

Epoxide Hydrolases
Adipose Tissue
Cardiovascular Abnormalities
Obesity
Adipocytes
Cardiovascular Diseases
Obese Mice
Peroxisome Proliferator-Activated Receptors
Intra-Abdominal Fat
Abdominal Obesity
Proteome
Inbred C57BL Mouse
Lipid Metabolism
Type 2 Diabetes Mellitus
Insulin Resistance
Public Health
Body Weight
Diet
Inflammation
Messenger RNA

ASJC Scopus subject areas

  • Endocrinology
  • Medicine (miscellaneous)
  • Endocrinology, Diabetes and Metabolism
  • Nutrition and Dietetics

Cite this

De Taeye, B. M., Morisseau, C., Coyle, J., Covington, J. W., Luria, A., Yang, J., ... Vaughan, D. E. (2010). Expression and regulation of soluble epoxide hydrolase in adipose tissue. Obesity, 18(3), 489-498. https://doi.org/10.1038/oby.2009.227

Expression and regulation of soluble epoxide hydrolase in adipose tissue. / De Taeye, Bart M.; Morisseau, Christophe; Coyle, Julie; Covington, Joseph W.; Luria, Ayala; Yang, Jun; Murphy, Sheila B.; Friedman, David B.; Hammock, Bruce B.; Vaughan, Douglas E.

In: Obesity, Vol. 18, No. 3, 03.2010, p. 489-498.

Research output: Contribution to journalArticle

De Taeye, BM, Morisseau, C, Coyle, J, Covington, JW, Luria, A, Yang, J, Murphy, SB, Friedman, DB, Hammock, BB & Vaughan, DE 2010, 'Expression and regulation of soluble epoxide hydrolase in adipose tissue', Obesity, vol. 18, no. 3, pp. 489-498. https://doi.org/10.1038/oby.2009.227
De Taeye BM, Morisseau C, Coyle J, Covington JW, Luria A, Yang J et al. Expression and regulation of soluble epoxide hydrolase in adipose tissue. Obesity. 2010 Mar;18(3):489-498. https://doi.org/10.1038/oby.2009.227
De Taeye, Bart M. ; Morisseau, Christophe ; Coyle, Julie ; Covington, Joseph W. ; Luria, Ayala ; Yang, Jun ; Murphy, Sheila B. ; Friedman, David B. ; Hammock, Bruce B. ; Vaughan, Douglas E. / Expression and regulation of soluble epoxide hydrolase in adipose tissue. In: Obesity. 2010 ; Vol. 18, No. 3. pp. 489-498.
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