Abstract
Obesity is an increasingly important public health issue reaching epidemic proportions. Visceral obesity has been defined as an important element of the metabolic syndrome and expansion of the visceral fat mass has been shown to contribute to the development of insulin resistance and cardiovascular disease. To identify novel contributors to cardiovascular and metabolic abnormalities in obesity, we analyzed the adipose proteome and identified soluble epoxide hydrolase (sEH) in the epididymal fat pad from C57BL/6J mice that received either a regular diet or a western diet. sEH was synthesized in adipocytes and expression levels increased upon differentiation of 3T3-L1 preadipocytes. Although normalized sEH mRNA and protein levels did not differ in the fat pads from mice receiving a regular or a western diet, total adipose sEH activity was higher in the obese mice, even after normalization for body weight. Furthermore, peroxisome proliferator-activated receptor γ (PPARγ) agonists increased the expression of sEH in mature 3T3-L1 adipocytes in vitro and in adipose tissue in vivo. Considering the established role for sEH in inflammation, cardiovascular diseases, and lipid metabolism, and the suggested involvement of sEH in the development of type 2 diabetes, our study has identified adipose sEH as a potential novel therapeutic target that might affect the development of metabolic and cardiovascular abnormalities in obesity.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 489-498 |
| Number of pages | 10 |
| Journal | Obesity |
| Volume | 18 |
| Issue number | 3 |
| DOIs | |
| State | Published - Mar 2010 |
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ASJC Scopus subject areas
- Endocrinology
- Medicine (miscellaneous)
- Endocrinology, Diabetes and Metabolism
- Nutrition and Dietetics
Cite this
Expression and regulation of soluble epoxide hydrolase in adipose tissue. / De Taeye, Bart M.; Morisseau, Christophe; Coyle, Julie; Covington, Joseph W.; Luria, Ayala; Yang, Jun; Murphy, Sheila B.; Friedman, David B.; Hammock, Bruce B.; Vaughan, Douglas E.
In: Obesity, Vol. 18, No. 3, 03.2010, p. 489-498.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Expression and regulation of soluble epoxide hydrolase in adipose tissue
AU - De Taeye, Bart M.
AU - Morisseau, Christophe
AU - Coyle, Julie
AU - Covington, Joseph W.
AU - Luria, Ayala
AU - Yang, Jun
AU - Murphy, Sheila B.
AU - Friedman, David B.
AU - Hammock, Bruce B.
AU - Vaughan, Douglas E.
PY - 2010/3
Y1 - 2010/3
N2 - Obesity is an increasingly important public health issue reaching epidemic proportions. Visceral obesity has been defined as an important element of the metabolic syndrome and expansion of the visceral fat mass has been shown to contribute to the development of insulin resistance and cardiovascular disease. To identify novel contributors to cardiovascular and metabolic abnormalities in obesity, we analyzed the adipose proteome and identified soluble epoxide hydrolase (sEH) in the epididymal fat pad from C57BL/6J mice that received either a regular diet or a western diet. sEH was synthesized in adipocytes and expression levels increased upon differentiation of 3T3-L1 preadipocytes. Although normalized sEH mRNA and protein levels did not differ in the fat pads from mice receiving a regular or a western diet, total adipose sEH activity was higher in the obese mice, even after normalization for body weight. Furthermore, peroxisome proliferator-activated receptor γ (PPARγ) agonists increased the expression of sEH in mature 3T3-L1 adipocytes in vitro and in adipose tissue in vivo. Considering the established role for sEH in inflammation, cardiovascular diseases, and lipid metabolism, and the suggested involvement of sEH in the development of type 2 diabetes, our study has identified adipose sEH as a potential novel therapeutic target that might affect the development of metabolic and cardiovascular abnormalities in obesity.
AB - Obesity is an increasingly important public health issue reaching epidemic proportions. Visceral obesity has been defined as an important element of the metabolic syndrome and expansion of the visceral fat mass has been shown to contribute to the development of insulin resistance and cardiovascular disease. To identify novel contributors to cardiovascular and metabolic abnormalities in obesity, we analyzed the adipose proteome and identified soluble epoxide hydrolase (sEH) in the epididymal fat pad from C57BL/6J mice that received either a regular diet or a western diet. sEH was synthesized in adipocytes and expression levels increased upon differentiation of 3T3-L1 preadipocytes. Although normalized sEH mRNA and protein levels did not differ in the fat pads from mice receiving a regular or a western diet, total adipose sEH activity was higher in the obese mice, even after normalization for body weight. Furthermore, peroxisome proliferator-activated receptor γ (PPARγ) agonists increased the expression of sEH in mature 3T3-L1 adipocytes in vitro and in adipose tissue in vivo. Considering the established role for sEH in inflammation, cardiovascular diseases, and lipid metabolism, and the suggested involvement of sEH in the development of type 2 diabetes, our study has identified adipose sEH as a potential novel therapeutic target that might affect the development of metabolic and cardiovascular abnormalities in obesity.
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UR - http://www.scopus.com/inward/citedby.url?scp=77249096825&partnerID=8YFLogxK
U2 - 10.1038/oby.2009.227
DO - 10.1038/oby.2009.227
M3 - Article
C2 - 19644452
AN - SCOPUS:77249096825
VL - 18
SP - 489
EP - 498
JO - Obesity
JF - Obesity
SN - 1930-7381
IS - 3
ER -