Expression and purification of functional human α-1-antitrypsin from cultured plant cells

J. Huang, T. D. Sutliff, L. Wu, S. Nandi, K. Benge, M. Terashima, A. H. Ralston, W. Drohan, N. Huang, R. L. Rodriguez

Research output: Contribution to journalArticle

81 Citations (Scopus)

Abstract

Human α-1-antitrypsin (AAT), the most abundant protease inhibitor found in the blood, was expressed in rice embryonic tissue suspension cell culture. This was accomplished by cloning the codon-optimized AAT gene into a vector containing the rice RAmy3D promoter and its signal sequence. The synthetic gene incorporates codons synonymous with those found in highly expressed rice genes. Approximately 1000 Stable transformed calli were produced by particle bombardment mediated transformation and were screened for high AAT expression using a porcine elastase inhibitory activity assay. The band shift assay also confirmed that rice-derived AAT is functional regarding its binding capability to the elastase substrate. Time course studies were conducted to determine the optimum, postinduction expression levels from cell culture. AAT expression equivalent to 20% of the total secreted proteins was achieved, and a purification scheme was developed that yielded active AAT with purity greater than 95%. The potential applications of purified plant-derived AAT for treatments of various AAT-deficient diseases are discussed.

Original languageEnglish (US)
Pages (from-to)126-133
Number of pages8
JournalBiotechnology Progress
Volume17
Issue number1
DOIs
StatePublished - 2001

Fingerprint

Plant Cells
Cultured Cells
rice
elastase
Pancreatic Elastase
codons
Codon
Cell Culture Techniques
cells
Synthetic Genes
synthetic genes
Bony Callus
assays
Protein Sorting Signals
proteinase inhibitors
signal peptide
Protease Inhibitors
purity
cell suspension culture
Genes

ASJC Scopus subject areas

  • Food Science
  • Biotechnology
  • Microbiology

Cite this

Huang, J., Sutliff, T. D., Wu, L., Nandi, S., Benge, K., Terashima, M., ... Rodriguez, R. L. (2001). Expression and purification of functional human α-1-antitrypsin from cultured plant cells. Biotechnology Progress, 17(1), 126-133. https://doi.org/10.1021/bp0001516

Expression and purification of functional human α-1-antitrypsin from cultured plant cells. / Huang, J.; Sutliff, T. D.; Wu, L.; Nandi, S.; Benge, K.; Terashima, M.; Ralston, A. H.; Drohan, W.; Huang, N.; Rodriguez, R. L.

In: Biotechnology Progress, Vol. 17, No. 1, 2001, p. 126-133.

Research output: Contribution to journalArticle

Huang, J, Sutliff, TD, Wu, L, Nandi, S, Benge, K, Terashima, M, Ralston, AH, Drohan, W, Huang, N & Rodriguez, RL 2001, 'Expression and purification of functional human α-1-antitrypsin from cultured plant cells', Biotechnology Progress, vol. 17, no. 1, pp. 126-133. https://doi.org/10.1021/bp0001516
Huang, J. ; Sutliff, T. D. ; Wu, L. ; Nandi, S. ; Benge, K. ; Terashima, M. ; Ralston, A. H. ; Drohan, W. ; Huang, N. ; Rodriguez, R. L. / Expression and purification of functional human α-1-antitrypsin from cultured plant cells. In: Biotechnology Progress. 2001 ; Vol. 17, No. 1. pp. 126-133.
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