Expression and function of an IgE-binding animal lectin (ε{lunate}BP) in mast cells

Fu-Tong Liu, Luciano G. Frigeri, Christine A. Gritzmacher, Daniel K. Hsu, Michael W. Robertson, Riaz I. Zuberi

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

ε{lunate}BP (IgE-binding protein) is a 31,000 Mr protein originally identified in rat basophilic leukemia (RBL) cells. The protein is composed of two domains with the amino-terminal domain containing a highly conserved repetitive sequence and the carboxyl-terminal domain containing consensus sequences shared by other β-galactoside-binding soluble lectins. The protein has wide tissue distribution, is found on cell surfaces and in extracellular milieu. By combined efforts from several research groups including ours a multifunctional nature of this lectin began to emerge. This review emphasizes the following characteristics of ε{lunate}BP: (i) ε{lunate}BP is secreted by cells such as macrophages; (ii) like many other lectins, ε{lunate}BP functions at least bivalently; (iii) ε{lunate}BP has specificity for distinct oligosaccharide structures that have a terminal galactose not masked by sialic acids; and (iv) in addition to binding IgE, ε{lunate}BP binds to surfaces of various cell types via lectin-carbohydrate interaction. Importantly, ε{lunate}BP binds to the IgE receptor on mast cells. We propose that ε{lunate}BP can function as a modulatory protein on various cells by cross-linking critical cell surface glycoproteins. The proposed action of ε{lunate}BP on mast cells is presented as a model.

Original languageEnglish (US)
Pages (from-to)187-195
Number of pages9
JournalImmunopharmacology
Volume26
Issue number3
DOIs
StatePublished - 1993

Fingerprint

Lectins
Mast Cells
Immunoglobulin E
Proteins
Sialic Acids
Galectin 3
IgE Receptors
Galactosides
Conserved Sequence
Nucleic Acid Repetitive Sequences
Membrane Glycoproteins
Consensus Sequence
Tissue Distribution
Oligosaccharides
Galactose
Leukemia
Macrophages
Carbohydrates
Research

Keywords

  • Animal lectin
  • Glycobiology
  • IgE
  • Mast cell
  • ε{lunate}-BP

ASJC Scopus subject areas

  • Pharmacology

Cite this

Liu, F-T., Frigeri, L. G., Gritzmacher, C. A., Hsu, D. K., Robertson, M. W., & Zuberi, R. I. (1993). Expression and function of an IgE-binding animal lectin (ε{lunate}BP) in mast cells. Immunopharmacology, 26(3), 187-195. https://doi.org/10.1016/0162-3109(93)90034-N

Expression and function of an IgE-binding animal lectin (ε{lunate}BP) in mast cells. / Liu, Fu-Tong; Frigeri, Luciano G.; Gritzmacher, Christine A.; Hsu, Daniel K.; Robertson, Michael W.; Zuberi, Riaz I.

In: Immunopharmacology, Vol. 26, No. 3, 1993, p. 187-195.

Research output: Contribution to journalArticle

Liu, F-T, Frigeri, LG, Gritzmacher, CA, Hsu, DK, Robertson, MW & Zuberi, RI 1993, 'Expression and function of an IgE-binding animal lectin (ε{lunate}BP) in mast cells', Immunopharmacology, vol. 26, no. 3, pp. 187-195. https://doi.org/10.1016/0162-3109(93)90034-N
Liu, Fu-Tong ; Frigeri, Luciano G. ; Gritzmacher, Christine A. ; Hsu, Daniel K. ; Robertson, Michael W. ; Zuberi, Riaz I. / Expression and function of an IgE-binding animal lectin (ε{lunate}BP) in mast cells. In: Immunopharmacology. 1993 ; Vol. 26, No. 3. pp. 187-195.
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