Abstract
Background. Olfactory discrimination depends on the large numbers of odorant receptor genes and differential ligand-receptor signaling among neurons expressing different receptors. In this study, we describe an in vitro system that enables the expression of exogenous odorant receptors in cultured olfactory sensory neurons. Olfactory sensory neurons in the culture express characteristic signaling molecules and, therefore, provide a system to study receptor function within its intrinsic cellular environment. Results. We demonstrate that cultured olfactory sensory neurons express endogenous odorant receptors. Lentiviral vector-mediated gene transfer enables successful ectopic expression of odorant receptors. We show that the ectopically expressed mouse I7 is functional in the cultured olfactory sensory neurons. When two different odorant receptors are ectopically expressed simultaneously, both receptor proteins co-localized in the same olfactory sensory neurons up to 10 days in vitro. Conclusion. This culture technique provided an efficient method to culture olfactory sensory neurons whose morphology, molecular characteristics and maturation progression resembled those observed in vivo. Using this system, regulation of odorant receptor expression and its ligand specificity can be studied in its intrinsic cellular environment.
| Original language | English (US) |
|---|---|
| Article number | 22 |
| Journal | Neural Development |
| Volume | 3 |
| Issue number | 1 |
| DOIs | |
| State | Published - 2008 |
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ASJC Scopus subject areas
- Developmental Neuroscience
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Expressing exogenous functional odorant receptors in cultured olfactory sensory neurons. / Chen, Huaiyang; Dadsetan, Sepehr; Fomina, Alla F; Gong, Qizhi.
In: Neural Development, Vol. 3, No. 1, 22, 2008.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Expressing exogenous functional odorant receptors in cultured olfactory sensory neurons
AU - Chen, Huaiyang
AU - Dadsetan, Sepehr
AU - Fomina, Alla F
AU - Gong, Qizhi
PY - 2008
Y1 - 2008
N2 - Background. Olfactory discrimination depends on the large numbers of odorant receptor genes and differential ligand-receptor signaling among neurons expressing different receptors. In this study, we describe an in vitro system that enables the expression of exogenous odorant receptors in cultured olfactory sensory neurons. Olfactory sensory neurons in the culture express characteristic signaling molecules and, therefore, provide a system to study receptor function within its intrinsic cellular environment. Results. We demonstrate that cultured olfactory sensory neurons express endogenous odorant receptors. Lentiviral vector-mediated gene transfer enables successful ectopic expression of odorant receptors. We show that the ectopically expressed mouse I7 is functional in the cultured olfactory sensory neurons. When two different odorant receptors are ectopically expressed simultaneously, both receptor proteins co-localized in the same olfactory sensory neurons up to 10 days in vitro. Conclusion. This culture technique provided an efficient method to culture olfactory sensory neurons whose morphology, molecular characteristics and maturation progression resembled those observed in vivo. Using this system, regulation of odorant receptor expression and its ligand specificity can be studied in its intrinsic cellular environment.
AB - Background. Olfactory discrimination depends on the large numbers of odorant receptor genes and differential ligand-receptor signaling among neurons expressing different receptors. In this study, we describe an in vitro system that enables the expression of exogenous odorant receptors in cultured olfactory sensory neurons. Olfactory sensory neurons in the culture express characteristic signaling molecules and, therefore, provide a system to study receptor function within its intrinsic cellular environment. Results. We demonstrate that cultured olfactory sensory neurons express endogenous odorant receptors. Lentiviral vector-mediated gene transfer enables successful ectopic expression of odorant receptors. We show that the ectopically expressed mouse I7 is functional in the cultured olfactory sensory neurons. When two different odorant receptors are ectopically expressed simultaneously, both receptor proteins co-localized in the same olfactory sensory neurons up to 10 days in vitro. Conclusion. This culture technique provided an efficient method to culture olfactory sensory neurons whose morphology, molecular characteristics and maturation progression resembled those observed in vivo. Using this system, regulation of odorant receptor expression and its ligand specificity can be studied in its intrinsic cellular environment.
UR - http://www.scopus.com/inward/record.url?scp=52249120382&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=52249120382&partnerID=8YFLogxK
U2 - 10.1186/1749-8104-3-22
DO - 10.1186/1749-8104-3-22
M3 - Article
C2 - 18786248
AN - SCOPUS:52249120382
VL - 3
JO - Neural Development
JF - Neural Development
SN - 1749-8104
IS - 1
M1 - 22
ER -