Exposure to tobacco smoke increases bone loss in spontaneously hypertensive rats

Jingyi Xu, Xing Qiu, Zhou Liang, Suzette Smiley-Jewell, Faqiang Lu, Mang Yu, Kent E Pinkerton, Dewei Zhao, Bingyin Shi

Research output: Contribution to journalArticle

Abstract

Purpose: To define if exposure to tobacco smoke (TS) could induce reduction of bone mass and impairment of bone architecture, features observed in osteoporosis in normotensive rats and the influence of TS exposure on the osteoporotic features exhibited in the spontaneously hypertensive (SH) rats. Methods: Normotensive Wistar Kyoto (WKY) and SH rats were exposed to filtered air or TS for 8 weeks, then their proximal femurs were extracted for micro-computed tomography (micro-CT) assessment, histological and immune-histological examinations to quantify the adverse influence of TS exposure on the bone mass and density, as well as bone architecture. Results: We found that TS exposure not only induced significant decreases in bone mineral density (BMD), bone volume (BV), cortical and trabecular thickness (Ct.Th and Tb.Th), trabecular surface area (Tb.Ar), expression of hypoxia-inducible factor-1α (HIF-1α) in the trabecular marrow, delayed ossification of cartilage, as well as statistical increases in trabecular separation (Tb.SP) and the number of trabecular marrow adipocytes in both WKY and SH rats, but also exacerbated multiple features of osteoporosis exhibited in SH rats, including decreased BMD, Ct.Th, Tb.Ar, HIF-1α expression, delayed cartilage ossification, and increased Tb.SP. Conclusions: Our results show that TS exposure can reduce bone mass and impair bone architecture and exacerbate multiple features of osteoporosis exhibited in SH rats.

Original languageEnglish (US)
JournalInhalation Toxicology
DOIs
StateAccepted/In press - Jan 1 2018

Fingerprint

Tobacco
Inbred SHR Rats
Smoke
Rats
Bone
Bone and Bones
Bone Density
Osteoporosis
Hypoxia-Inducible Factor 1
Osteogenesis
Cartilage
Bone Marrow
Minerals
Adipocytes
Femur
Air
Tomography

Keywords

  • bone architecture
  • bone loss
  • Femur
  • spontaneously hypertensive
  • tobacco smoking exposure

ASJC Scopus subject areas

  • Toxicology
  • Health, Toxicology and Mutagenesis

Cite this

Xu, J., Qiu, X., Liang, Z., Smiley-Jewell, S., Lu, F., Yu, M., ... Shi, B. (Accepted/In press). Exposure to tobacco smoke increases bone loss in spontaneously hypertensive rats. Inhalation Toxicology. https://doi.org/10.1080/08958378.2018.1506838

Exposure to tobacco smoke increases bone loss in spontaneously hypertensive rats. / Xu, Jingyi; Qiu, Xing; Liang, Zhou; Smiley-Jewell, Suzette; Lu, Faqiang; Yu, Mang; Pinkerton, Kent E; Zhao, Dewei; Shi, Bingyin.

In: Inhalation Toxicology, 01.01.2018.

Research output: Contribution to journalArticle

Xu, Jingyi ; Qiu, Xing ; Liang, Zhou ; Smiley-Jewell, Suzette ; Lu, Faqiang ; Yu, Mang ; Pinkerton, Kent E ; Zhao, Dewei ; Shi, Bingyin. / Exposure to tobacco smoke increases bone loss in spontaneously hypertensive rats. In: Inhalation Toxicology. 2018.
@article{d14f7637a12e4514b12392309be7b966,
title = "Exposure to tobacco smoke increases bone loss in spontaneously hypertensive rats",
abstract = "Purpose: To define if exposure to tobacco smoke (TS) could induce reduction of bone mass and impairment of bone architecture, features observed in osteoporosis in normotensive rats and the influence of TS exposure on the osteoporotic features exhibited in the spontaneously hypertensive (SH) rats. Methods: Normotensive Wistar Kyoto (WKY) and SH rats were exposed to filtered air or TS for 8 weeks, then their proximal femurs were extracted for micro-computed tomography (micro-CT) assessment, histological and immune-histological examinations to quantify the adverse influence of TS exposure on the bone mass and density, as well as bone architecture. Results: We found that TS exposure not only induced significant decreases in bone mineral density (BMD), bone volume (BV), cortical and trabecular thickness (Ct.Th and Tb.Th), trabecular surface area (Tb.Ar), expression of hypoxia-inducible factor-1α (HIF-1α) in the trabecular marrow, delayed ossification of cartilage, as well as statistical increases in trabecular separation (Tb.SP) and the number of trabecular marrow adipocytes in both WKY and SH rats, but also exacerbated multiple features of osteoporosis exhibited in SH rats, including decreased BMD, Ct.Th, Tb.Ar, HIF-1α expression, delayed cartilage ossification, and increased Tb.SP. Conclusions: Our results show that TS exposure can reduce bone mass and impair bone architecture and exacerbate multiple features of osteoporosis exhibited in SH rats.",
keywords = "bone architecture, bone loss, Femur, spontaneously hypertensive, tobacco smoking exposure",
author = "Jingyi Xu and Xing Qiu and Zhou Liang and Suzette Smiley-Jewell and Faqiang Lu and Mang Yu and Pinkerton, {Kent E} and Dewei Zhao and Bingyin Shi",
year = "2018",
month = "1",
day = "1",
doi = "10.1080/08958378.2018.1506838",
language = "English (US)",
journal = "Inhalation Toxicology",
issn = "0895-8378",
publisher = "Informa Healthcare",

}

TY - JOUR

T1 - Exposure to tobacco smoke increases bone loss in spontaneously hypertensive rats

AU - Xu, Jingyi

AU - Qiu, Xing

AU - Liang, Zhou

AU - Smiley-Jewell, Suzette

AU - Lu, Faqiang

AU - Yu, Mang

AU - Pinkerton, Kent E

AU - Zhao, Dewei

AU - Shi, Bingyin

PY - 2018/1/1

Y1 - 2018/1/1

N2 - Purpose: To define if exposure to tobacco smoke (TS) could induce reduction of bone mass and impairment of bone architecture, features observed in osteoporosis in normotensive rats and the influence of TS exposure on the osteoporotic features exhibited in the spontaneously hypertensive (SH) rats. Methods: Normotensive Wistar Kyoto (WKY) and SH rats were exposed to filtered air or TS for 8 weeks, then their proximal femurs were extracted for micro-computed tomography (micro-CT) assessment, histological and immune-histological examinations to quantify the adverse influence of TS exposure on the bone mass and density, as well as bone architecture. Results: We found that TS exposure not only induced significant decreases in bone mineral density (BMD), bone volume (BV), cortical and trabecular thickness (Ct.Th and Tb.Th), trabecular surface area (Tb.Ar), expression of hypoxia-inducible factor-1α (HIF-1α) in the trabecular marrow, delayed ossification of cartilage, as well as statistical increases in trabecular separation (Tb.SP) and the number of trabecular marrow adipocytes in both WKY and SH rats, but also exacerbated multiple features of osteoporosis exhibited in SH rats, including decreased BMD, Ct.Th, Tb.Ar, HIF-1α expression, delayed cartilage ossification, and increased Tb.SP. Conclusions: Our results show that TS exposure can reduce bone mass and impair bone architecture and exacerbate multiple features of osteoporosis exhibited in SH rats.

AB - Purpose: To define if exposure to tobacco smoke (TS) could induce reduction of bone mass and impairment of bone architecture, features observed in osteoporosis in normotensive rats and the influence of TS exposure on the osteoporotic features exhibited in the spontaneously hypertensive (SH) rats. Methods: Normotensive Wistar Kyoto (WKY) and SH rats were exposed to filtered air or TS for 8 weeks, then their proximal femurs were extracted for micro-computed tomography (micro-CT) assessment, histological and immune-histological examinations to quantify the adverse influence of TS exposure on the bone mass and density, as well as bone architecture. Results: We found that TS exposure not only induced significant decreases in bone mineral density (BMD), bone volume (BV), cortical and trabecular thickness (Ct.Th and Tb.Th), trabecular surface area (Tb.Ar), expression of hypoxia-inducible factor-1α (HIF-1α) in the trabecular marrow, delayed ossification of cartilage, as well as statistical increases in trabecular separation (Tb.SP) and the number of trabecular marrow adipocytes in both WKY and SH rats, but also exacerbated multiple features of osteoporosis exhibited in SH rats, including decreased BMD, Ct.Th, Tb.Ar, HIF-1α expression, delayed cartilage ossification, and increased Tb.SP. Conclusions: Our results show that TS exposure can reduce bone mass and impair bone architecture and exacerbate multiple features of osteoporosis exhibited in SH rats.

KW - bone architecture

KW - bone loss

KW - Femur

KW - spontaneously hypertensive

KW - tobacco smoking exposure

UR - http://www.scopus.com/inward/record.url?scp=85053933521&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85053933521&partnerID=8YFLogxK

U2 - 10.1080/08958378.2018.1506838

DO - 10.1080/08958378.2018.1506838

M3 - Article

C2 - 30257116

AN - SCOPUS:85053933521

JO - Inhalation Toxicology

JF - Inhalation Toxicology

SN - 0895-8378

ER -