Exposure to sidestream cigarette smoke alters bronchiolar epithelial cell differentiation in the postnatal rat lung.

C. M. Ji, Charles Plopper, H. P. Witschi, Kent E Pinkerton

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

This study examines the effects of an ambient concentration of aged and diluted sidestream cigarette smoke (ADSS) on bronchiolar epithelial cell development and the expression of cytochrome P450 isozyme 1A1 protein in the postnatal rat lung. In control animals, the labeling indices for epithelial cells in proximal bronchi and terminal bronchioles at 7 days of age were 2.3 and 4.9%, respectively, and decreased to 0.1 and 0%, by 100 days of age. With exposure to ADSS from birth, the labeling index of epithelial cells in distal airways of rats was significantly reduced at 7 and 14 days of age, but not in epithelial cells of proximal bronchi. The expression of P450 isozyme 1A1 antigen in bronchiolar epithelial cells of control rats reached the maximal level observed at 21 days of age and subsequently decreased to low levels at 50 and 100 days of age. In contrast, exposure to ADSS significantly increased the distribution and intensity of staining for 1A1 antigen in bronchiolar epithelial cells of proximal and distal airways as early as 7 days of age and maintained elevated levels of 1A1 protein in these cells through 100 days of age. At 21 and 50 days of age, NADPH reductase protein expression was higher in the airway epithelium of rats exposed from birth to ADSS than that noted in the airways of controls. In contrast, cytochrome P450 isozyme 2B and Clara cell secretory protein expression were unchanged.(ABSTRACT TRUNCATED AT 250 WORDS)

Original languageEnglish (US)
Pages (from-to)312-320
Number of pages9
JournalAmerican Journal of Respiratory Cell and Molecular Biology
Volume11
Issue number3
StatePublished - Sep 1994

Fingerprint

Smoke
Tobacco Products
Rats
Cell Differentiation
Epithelial Cells
Lung
Isoenzymes
Bronchi
Cytochrome P-450 Enzyme System
Labeling
Uteroglobin
Rat control
Parturition
Bronchioles
Antigens
Proteins
Airway Management
NADP
Oxidoreductases
Animals

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology
  • Pulmonary and Respiratory Medicine

Cite this

@article{c19562dbcc4c4f03bd72313558a171d2,
title = "Exposure to sidestream cigarette smoke alters bronchiolar epithelial cell differentiation in the postnatal rat lung.",
abstract = "This study examines the effects of an ambient concentration of aged and diluted sidestream cigarette smoke (ADSS) on bronchiolar epithelial cell development and the expression of cytochrome P450 isozyme 1A1 protein in the postnatal rat lung. In control animals, the labeling indices for epithelial cells in proximal bronchi and terminal bronchioles at 7 days of age were 2.3 and 4.9{\%}, respectively, and decreased to 0.1 and 0{\%}, by 100 days of age. With exposure to ADSS from birth, the labeling index of epithelial cells in distal airways of rats was significantly reduced at 7 and 14 days of age, but not in epithelial cells of proximal bronchi. The expression of P450 isozyme 1A1 antigen in bronchiolar epithelial cells of control rats reached the maximal level observed at 21 days of age and subsequently decreased to low levels at 50 and 100 days of age. In contrast, exposure to ADSS significantly increased the distribution and intensity of staining for 1A1 antigen in bronchiolar epithelial cells of proximal and distal airways as early as 7 days of age and maintained elevated levels of 1A1 protein in these cells through 100 days of age. At 21 and 50 days of age, NADPH reductase protein expression was higher in the airway epithelium of rats exposed from birth to ADSS than that noted in the airways of controls. In contrast, cytochrome P450 isozyme 2B and Clara cell secretory protein expression were unchanged.(ABSTRACT TRUNCATED AT 250 WORDS)",
author = "Ji, {C. M.} and Charles Plopper and Witschi, {H. P.} and Pinkerton, {Kent E}",
year = "1994",
month = "9",
language = "English (US)",
volume = "11",
pages = "312--320",
journal = "American Journal of Respiratory Cell and Molecular Biology",
issn = "1044-1549",
publisher = "American Thoracic Society",
number = "3",

}

TY - JOUR

T1 - Exposure to sidestream cigarette smoke alters bronchiolar epithelial cell differentiation in the postnatal rat lung.

AU - Ji, C. M.

AU - Plopper, Charles

AU - Witschi, H. P.

AU - Pinkerton, Kent E

PY - 1994/9

Y1 - 1994/9

N2 - This study examines the effects of an ambient concentration of aged and diluted sidestream cigarette smoke (ADSS) on bronchiolar epithelial cell development and the expression of cytochrome P450 isozyme 1A1 protein in the postnatal rat lung. In control animals, the labeling indices for epithelial cells in proximal bronchi and terminal bronchioles at 7 days of age were 2.3 and 4.9%, respectively, and decreased to 0.1 and 0%, by 100 days of age. With exposure to ADSS from birth, the labeling index of epithelial cells in distal airways of rats was significantly reduced at 7 and 14 days of age, but not in epithelial cells of proximal bronchi. The expression of P450 isozyme 1A1 antigen in bronchiolar epithelial cells of control rats reached the maximal level observed at 21 days of age and subsequently decreased to low levels at 50 and 100 days of age. In contrast, exposure to ADSS significantly increased the distribution and intensity of staining for 1A1 antigen in bronchiolar epithelial cells of proximal and distal airways as early as 7 days of age and maintained elevated levels of 1A1 protein in these cells through 100 days of age. At 21 and 50 days of age, NADPH reductase protein expression was higher in the airway epithelium of rats exposed from birth to ADSS than that noted in the airways of controls. In contrast, cytochrome P450 isozyme 2B and Clara cell secretory protein expression were unchanged.(ABSTRACT TRUNCATED AT 250 WORDS)

AB - This study examines the effects of an ambient concentration of aged and diluted sidestream cigarette smoke (ADSS) on bronchiolar epithelial cell development and the expression of cytochrome P450 isozyme 1A1 protein in the postnatal rat lung. In control animals, the labeling indices for epithelial cells in proximal bronchi and terminal bronchioles at 7 days of age were 2.3 and 4.9%, respectively, and decreased to 0.1 and 0%, by 100 days of age. With exposure to ADSS from birth, the labeling index of epithelial cells in distal airways of rats was significantly reduced at 7 and 14 days of age, but not in epithelial cells of proximal bronchi. The expression of P450 isozyme 1A1 antigen in bronchiolar epithelial cells of control rats reached the maximal level observed at 21 days of age and subsequently decreased to low levels at 50 and 100 days of age. In contrast, exposure to ADSS significantly increased the distribution and intensity of staining for 1A1 antigen in bronchiolar epithelial cells of proximal and distal airways as early as 7 days of age and maintained elevated levels of 1A1 protein in these cells through 100 days of age. At 21 and 50 days of age, NADPH reductase protein expression was higher in the airway epithelium of rats exposed from birth to ADSS than that noted in the airways of controls. In contrast, cytochrome P450 isozyme 2B and Clara cell secretory protein expression were unchanged.(ABSTRACT TRUNCATED AT 250 WORDS)

UR - http://www.scopus.com/inward/record.url?scp=0028504578&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028504578&partnerID=8YFLogxK

M3 - Article

C2 - 8086168

AN - SCOPUS:0028504578

VL - 11

SP - 312

EP - 320

JO - American Journal of Respiratory Cell and Molecular Biology

JF - American Journal of Respiratory Cell and Molecular Biology

SN - 1044-1549

IS - 3

ER -