Exposure to environmental tobacco smoke induces angiogenesis and leukocyte trafficking in lung microvessels

Savita P. Rao, Lyudmila Sikora, M. Reza Hosseinkhani, Kent E Pinkerton, P. Sriramarao

Research output: Contribution to journalArticle

17 Scopus citations

Abstract

Exposure to environmental tobacco smoke (ETS) is known to contribute to and exacerbate inflammatory diseases of the lung such as chronic obstructive pulmonary disease (COPD) and asthma. The effect of ETS on angiogenesis and leukocyte recruitment, both of which promote lung inflammation, was investigated using lung tissue from mice exposed to aged and diluted sidestream cigarette smoke or fresh air for 12 weeks and transplanted into dorsal skin-fold chambers in nude mice. Lung tissue from mice exposed to cigarette smoke for 12 weeks exhibited significantly increased vascular density (angiogenesis) associated with selectin-mediated increased intravascular leukocyte rolling and adhesion compared to fresh air-exposed lung tissue by intravital microscopy. Further, neutrophils from nicotine-exposed mice displayed significantly increased rolling and adhesion compared to control neutrophils in microvessels of nicotine-exposed lungs versus control lung microvessels, suggesting that nicotine in cigarette smoke can augment leukocyte-endothelial interactions. ETS-induced angiogenesis and leukocyte trafficking may play a key role in airway recruitment of inflammatory cells in ETS-associated disorders such as COPD bronchitis or asthma.

Original languageEnglish (US)
Pages (from-to)119-135
Number of pages17
JournalExperimental Lung Research
Volume35
Issue number2
DOIs
StatePublished - Mar 2009

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Keywords

  • Environmental tobacco smoke
  • Leukocyte trafficking
  • Lung angiogenesis
  • Nicotine

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine
  • Molecular Biology
  • Clinical Biochemistry

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