Exposure of cynomolgus monkey embryos to retinoic acid causes thymic defects: Effects on peripheral lymphoid organ development

N. Makori, P. E. Peterson, K. Lantz, Andrew G Hendrickx

Research output: Contribution to journalArticle

8 Scopus citations

Abstract

We have previously reported that exposure of monkey embryos to 13-cis-retinoic acid (cRA) results in thymic defects. In this study, we analyzed lymphocyte and antigen-presenting cell populations at gestational days (GDs) 80-100 in the thymus, spleen, mesenteric lymph nodes, and gut-associated lymphoid tissue following a teratogenic dosing regimen of cRA (2.5 and 5 mg/kg) at GD14-27. Tissue sections were immunostained for T-cells (anti-CD3), B-cells (anti-CD20), dendritic cells (p55), and major histocompatibility class II (anti-HLA-DR). Digital images of spleen sections were analyzed to obtain the relative area occupied by the cell subsets within the white pulp (WP). Compared with controls, the T-cell dependent compartment of the spleen WP in specimens with perturbed thymic development (aplasia and severe hypoplasia) showed a reduction in size and proportion of CD3+ T cells. Our findings indicate that cRA-induced thymic defects result in disrupted development of the splenic T-cell dependent compartment.

Original languageEnglish (US)
Pages (from-to)91-97
Number of pages7
JournalJournal of Medical Primatology
Volume31
Issue number2
DOIs
StatePublished - 2002

Keywords

  • Fetus
  • Immunotoxicity
  • Macaca fascicularis
  • Spleen
  • T- and B-lymphocytes

ASJC Scopus subject areas

  • Animal Science and Zoology
  • veterinary(all)

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