Exploring the Transcriptome Landscape of Pomegranate Fruit Peel for Natural Product Biosynthetic Gene and SSR Marker Discovery

Nadia Nicole Ono, Monica Therese Britton, Joseph Nathaniel Fass, Charles Meyer Nicolet, Dawei Lin, Li Tian

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

Pomegranate fruit peel is rich in bioactive plant natural products, such as hydrolyzable tannins and anthocyanins. Despite their documented roles in human nutrition and fruit quality, genes involved in natural product biosynthesis have not been cloned from pomegranate and very little sequence information is available on pomegranate in the public domain. Shotgun transcriptome sequencing of pomegranate fruit peel cDNA was performed using RNA-Seq on the Illumina Genome Analyzer platform. Over 100 million raw sequence reads were obtained and assembled into 9,839 transcriptome assemblies (TAs) (>200 bp). Candidate genes for hydrolyzable tannin, anthocyanin, flavonoid, terpenoid and fatty acid biosynthesis and/or regulation were identified. Three lipid transfer proteins were obtained that may contribute to the previously reported IgE reactivity of pomegranate fruit extracts. In addition, 115 SSR markers were identified from the pomegranate fruit peel transcriptome and primers were designed for 77 SSR markers. The pomegranate fruit peel transcriptome set provides a valuable platform for natural product biosynthetic gene and SSR marker discovery in pomegranate. This work also demonstrates that next-generation transcriptome sequencing is an economical and effective approach for investigating natural product biosynthesis, identifying genes controlling important agronomic traits, and discovering molecular markers in non-model specialty crop species.

Original languageEnglish (US)
Pages (from-to)800-813
Number of pages14
JournalJournal of Integrative Plant Biology
Volume53
Issue number10
DOIs
StatePublished - Oct 2011

Fingerprint

Punicaceae
fruit peels
Fruits
Biological Products
Transcriptome
transcriptome
Fruit
Genes
Biosynthesis
biosynthesis
transcriptomics
Hydrolyzable Tannins
tannins
anthocyanins
genes
Anthocyanins
specialty crops
fruit extracts
human nutrition
agronomic traits

Keywords

  • Hydrolyzable tannin
  • Natural product
  • Pomegranate
  • SSR marker
  • Transcriptome

ASJC Scopus subject areas

  • Plant Science
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry

Cite this

Exploring the Transcriptome Landscape of Pomegranate Fruit Peel for Natural Product Biosynthetic Gene and SSR Marker Discovery. / Ono, Nadia Nicole; Britton, Monica Therese; Fass, Joseph Nathaniel; Nicolet, Charles Meyer; Lin, Dawei; Tian, Li.

In: Journal of Integrative Plant Biology, Vol. 53, No. 10, 10.2011, p. 800-813.

Research output: Contribution to journalArticle

Ono, Nadia Nicole ; Britton, Monica Therese ; Fass, Joseph Nathaniel ; Nicolet, Charles Meyer ; Lin, Dawei ; Tian, Li. / Exploring the Transcriptome Landscape of Pomegranate Fruit Peel for Natural Product Biosynthetic Gene and SSR Marker Discovery. In: Journal of Integrative Plant Biology. 2011 ; Vol. 53, No. 10. pp. 800-813.
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