Exploring reaction pathways of single-molecule interactions through the manipulation and tracking of a potential-confined microsphere in three dimensions

Wesley P. Wong, Volkmar Heinrich, Evan Evans

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Scopus citations

Abstract

A single-bond dynamics by tracking brownian fluctuations of functionalized bead was discussed. A weak optical-trapping potential was used to confine motion of the bead to a nanoscale domain and to apply a controlled bias field to the interaction. Variations in the motion of a bead linked to the substrate via a biomolecule single conformational changes in the molecule such as the folding/unfolding of protein domains or the unzipping of DNA. The results show that bonding events between molecules immobilized on the bead and on the substrate can be detected with clear discrimination of single-molecule attachments.

Original languageEnglish (US)
Title of host publicationMaterials Research Society Symposium - Proceedings
EditorsJ.T. Fourkas, P. Levitz, M. Urbakh, K.J. Wahl
Pages125-136
Number of pages12
Volume790
StatePublished - 2003
Externally publishedYes
EventDynamics in Small Confining Systems - 2003 - Boston, MA, United States
Duration: Dec 1 2003Dec 4 2003

Other

OtherDynamics in Small Confining Systems - 2003
CountryUnited States
CityBoston, MA
Period12/1/0312/4/03

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials

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    Wong, W. P., Heinrich, V., & Evans, E. (2003). Exploring reaction pathways of single-molecule interactions through the manipulation and tracking of a potential-confined microsphere in three dimensions. In J. T. Fourkas, P. Levitz, M. Urbakh, & K. J. Wahl (Eds.), Materials Research Society Symposium - Proceedings (Vol. 790, pp. 125-136)