Exploring bacterial epigenomics in the next-generation sequencing era: A new approach for an emerging frontier

Poyin Chen, Richard Jeannotte, Bart C Weimer

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Epigenetics has an important role for the success of foodborne pathogen persistence in diverse host niches. Substantial challenges exist in determining DNA methylation to situation-specific phenotypic traits. DNA modification, mediated by restriction-modification systems, functions as an immune response against antagonistic external DNA, and bacteriophage-acquired methyltransferases (MTase) and orphan MTases - those lacking the cognate restriction endonuclease - facilitate evolution of new phenotypes via gene expression modulation via DNA and RNA modifications, including methylation and phosphorothioation. Recent establishment of large-scale genome sequencing projects will result in a significant increase in genome availability that will lead to new demands for data analysis including new predictive bioinformatics approaches that can be verified with traditional scientific rigor. Sequencing technologies that detect modification coupled with mass spectrometry to discover new adducts is a powerful tactic to study bacterial epigenetics, which is poised to make novel and far-reaching discoveries that link biological significance and the bacterial epigenome.

Original languageEnglish (US)
Pages (from-to)292-300
Number of pages9
JournalTrends in Microbiology
Volume22
Issue number5
DOIs
StatePublished - 2014

Fingerprint

Epigenomics
DNA
DNA Restriction-Modification Enzymes
Genome
DNA Restriction Enzymes
Methyltransferases
DNA Methylation
Computational Biology
Bacteriophages
Methylation
Mass Spectrometry
RNA
Technology
Phenotype
Gene Expression

Keywords

  • DNA adduct
  • Epigenetics
  • Methylation
  • Multi-omics
  • Phosphorothioation

ASJC Scopus subject areas

  • Infectious Diseases
  • Microbiology (medical)
  • Microbiology
  • Virology
  • Medicine(all)

Cite this

Exploring bacterial epigenomics in the next-generation sequencing era : A new approach for an emerging frontier. / Chen, Poyin; Jeannotte, Richard; Weimer, Bart C.

In: Trends in Microbiology, Vol. 22, No. 5, 2014, p. 292-300.

Research output: Contribution to journalArticle

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