Exploration of polar lipid accumulation profiles in Euglena gracilis using LipidBlast, an MS/MS spectral library constructed in silico

Takumi Ogawa; Takeshi Furuhashi; Atsushi Okazawa; Rai Nakai; Masami Nakazawa; Tobias Kind; Oliver Fiehn; Shigehiko Kanaya; Masanori Arita; Daisaku Ohta

doi:10.1080/09168451.2014.877826

Exploration of polar lipid accumulation profiles in Euglena gracilis using LipidBlast, an MS/MS spectral library constructed in silico

Takumi Ogawa, Takeshi Furuhashi, Atsushi Okazawa, Rai Nakai, Masami Nakazawa, Tobias Kind, Oliver Fiehn, Shigehiko Kanaya, Masanori Arita, Daisaku Ohta

Molecular and Cellular Biology

Research output: Contribution to journal › Article

5 Scopus citations

Abstract

A rapid protocol for polar lipid profiling was applied to Euglena gracilis lipid metabolism by LipidBlast, an MS/MS spectral similarity search tool. The similarity search results suggested anoxia-induced polar lipid metabolism in Euglena characterized by the accumulation of differential lipid classes, carbon chain lengths, and unsaturated bond numbers. The informatics-supported MS spectral search provides an alternative option for global lipid profiling studies.

Original language	English (US)
Pages (from-to)	14-18
Number of pages	5
Journal	Bioscience, biotechnology, and biochemistry
Volume	78
Issue number	1
DOIs	https://doi.org/10.1080/09168451.2014.877826
State	Published - 2014

Keywords

Euglena gracilis
LC–MS/MS
LipidBlast
polar lipid profiling

ASJC Scopus subject areas

Medicine(all)

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10.1080/09168451.2014.877826

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Ogawa, T., Furuhashi, T., Okazawa, A., Nakai, R., Nakazawa, M., Kind, T., Fiehn, O., Kanaya, S., Arita, M., & Ohta, D. (2014). Exploration of polar lipid accumulation profiles in Euglena gracilis using LipidBlast, an MS/MS spectral library constructed in silico. Bioscience, biotechnology, and biochemistry, 78(1), 14-18. https://doi.org/10.1080/09168451.2014.877826

Exploration of polar lipid accumulation profiles in Euglena gracilis using LipidBlast, an MS/MS spectral library constructed in silico. / Ogawa, Takumi; Furuhashi, Takeshi; Okazawa, Atsushi; Nakai, Rai; Nakazawa, Masami; Kind, Tobias; Fiehn, Oliver; Kanaya, Shigehiko; Arita, Masanori; Ohta, Daisaku.

In: Bioscience, biotechnology, and biochemistry, Vol. 78, No. 1, 2014, p. 14-18.

Research output: Contribution to journal › Article

Ogawa, Takumi ; Furuhashi, Takeshi ; Okazawa, Atsushi ; Nakai, Rai ; Nakazawa, Masami ; Kind, Tobias ; Fiehn, Oliver ; Kanaya, Shigehiko ; Arita, Masanori ; Ohta, Daisaku. / Exploration of polar lipid accumulation profiles in Euglena gracilis using LipidBlast, an MS/MS spectral library constructed in silico. In: Bioscience, biotechnology, and biochemistry. 2014 ; Vol. 78, No. 1. pp. 14-18.

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