Exploration of polar lipid accumulation profiles in Euglena gracilis using LipidBlast, an MS/MS spectral library constructed in silico

Takumi Ogawa, Takeshi Furuhashi, Atsushi Okazawa, Rai Nakai, Masami Nakazawa, Tobias Kind, Oliver Fiehn, Shigehiko Kanaya, Masanori Arita, Daisaku Ohta

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

A rapid protocol for polar lipid profiling was applied to Euglena gracilis lipid metabolism by LipidBlast, an MS/MS spectral similarity search tool. The similarity search results suggested anoxia-induced polar lipid metabolism in Euglena characterized by the accumulation of differential lipid classes, carbon chain lengths, and unsaturated bond numbers. The informatics-supported MS spectral search provides an alternative option for global lipid profiling studies.

Original languageEnglish (US)
Pages (from-to)14-18
Number of pages5
JournalBioscience, biotechnology, and biochemistry
Volume78
Issue number1
DOIs
StatePublished - 2014

Keywords

  • Euglena gracilis
  • LC–MS/MS
  • LipidBlast
  • polar lipid profiling

ASJC Scopus subject areas

  • Medicine(all)

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