Exploration of polar lipid accumulation profiles in Euglena gracilis using LipidBlast, an MS/MS spectral library constructed in silico

Takumi Ogawa, Takeshi Furuhashi, Atsushi Okazawa, Rai Nakai, Masami Nakazawa, Tobias Kind, Oliver Fiehn, Shigehiko Kanaya, Masanori Arita, Daisaku Ohta

Research output: Contribution to journal › Article

5 Citations (Scopus)

Abstract

A rapid protocol for polar lipid profiling was applied to Euglena gracilis lipid metabolism by LipidBlast, an MS/MS spectral similarity search tool. The similarity search results suggested anoxia-induced polar lipid metabolism in Euglena characterized by the accumulation of differential lipid classes, carbon chain lengths, and unsaturated bond numbers. The informatics-supported MS spectral search provides an alternative option for global lipid profiling studies.

Original languageEnglish (US)
Pages (from-to)14-18
Number of pages5
JournalBioscience, biotechnology, and biochemistry
Volume78
Issue number1
DOIs
StatePublished - 2014

Fingerprint

Euglena gracilis
Computer Simulation
Libraries
Lipids
Lipid Metabolism
Euglena
Informatics
Chain length
Carbon

Keywords

  • Euglena gracilis
  • LC–MS/MS
  • LipidBlast
  • polar lipid profiling

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Exploration of polar lipid accumulation profiles in Euglena gracilis using LipidBlast, an MS/MS spectral library constructed in silico. / Ogawa, Takumi; Furuhashi, Takeshi; Okazawa, Atsushi; Nakai, Rai; Nakazawa, Masami; Kind, Tobias; Fiehn, Oliver; Kanaya, Shigehiko; Arita, Masanori; Ohta, Daisaku.

In: Bioscience, biotechnology, and biochemistry, Vol. 78, No. 1, 2014, p. 14-18.

Research output: Contribution to journal › Article

Ogawa, T, Furuhashi, T, Okazawa, A, Nakai, R, Nakazawa, M, Kind, T, Fiehn, O, Kanaya, S, Arita, M & Ohta, D 2014, 'Exploration of polar lipid accumulation profiles in Euglena gracilis using LipidBlast, an MS/MS spectral library constructed in silico', Bioscience, biotechnology, and biochemistry, vol. 78, no. 1, pp. 14-18. https://doi.org/10.1080/09168451.2014.877826
Ogawa T, Furuhashi T, Okazawa A, Nakai R, Nakazawa M, Kind T et al. Exploration of polar lipid accumulation profiles in Euglena gracilis using LipidBlast, an MS/MS spectral library constructed in silico. Bioscience, biotechnology, and biochemistry. 2014;78(1):14-18. https://doi.org/10.1080/09168451.2014.877826
Ogawa, Takumi ; Furuhashi, Takeshi ; Okazawa, Atsushi ; Nakai, Rai ; Nakazawa, Masami ; Kind, Tobias ; Fiehn, Oliver ; Kanaya, Shigehiko ; Arita, Masanori ; Ohta, Daisaku. / Exploration of polar lipid accumulation profiles in Euglena gracilis using LipidBlast, an MS/MS spectral library constructed in silico. In: Bioscience, biotechnology, and biochemistry. 2014 ; Vol. 78, No. 1. pp. 14-18.
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