Experimental transmission of Bartonella henselae by the cat flea

Bruno B Chomel, Rickie W. Kasten, Kim Floyd-Hawkins, Banghee Chi, Kazuhiro Yamamoto, Jill Roberts-Wilson, A. Nikos Gurfield, Rachel C. Abbott, Niels C Pedersen, Jane E. Koehler

Research output: Contribution to journalArticle

432 Citations (Scopus)

Abstract

Bartonella henselae is an emerging bacterial pathogen, causing cat scratch disease and bacillary angiomatosis. Cats bacteremic with B. henselae constitute a large reservoir from which humans become infected. Prevention of human infection depends on elucidation of the natural history and means of feline infection. We studied 47 cattery cats in a private home for 12 months to determine the longitudinal prevalence of B. henselae bacteremia, the prevalence of B. henselae in the fleas infesting these cats, and whether B. henselae is transmitted experimentally to cats via fleas. Vector-mediated transmission of B. henselae isolates was evaluated by removing fleas from the naturally bacteremic, flea-infested cattery cats and transferring these fleas to specific-pathogen-free (SPF) kittens housed in a controlled, arthropod- free University Animal Facility. B. henselae bacteremia was detected in 89% of the 47 naturally infected cattery cats. A total of 132 fleas were removed from cats whose blood was simultaneously cultured during different seasons and were tested individually for the presence of B. henselae DNA by PCR. B. henselae DNA was detected in 34% of 132 fleas, with seasonal variation, but without an association between the presence or the level of bacteremia in the corresponding cat. Cat fleas removed from bacteremic cattery cats transmitted B. henselae to five SPF kittens in two separate experiments; however, control SPF kittens ho used with highly bacteremic kittens in the absence of fleas did not become infected. These data demonstrate that the cat flea readily transmits B. henselae to cats. Control of feline infestation with this arthropod vector may provide an important strategy for the prevention of infection of both humans and cats.

Original languageEnglish (US)
Pages (from-to)1952-1956
Number of pages5
JournalJournal of Clinical Microbiology
Volume34
Issue number8
StatePublished - Aug 1996

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Ctenocephalides
Bartonella henselae
Siphonaptera
Cats
Specific Pathogen-Free Organisms
Bacteremia
Felidae
Bacillary Angiomatosis
Arthropod Vectors
Infection
Cat-Scratch Disease
Arthropods
DNA
Natural History

ASJC Scopus subject areas

  • Microbiology (medical)
  • Microbiology

Cite this

Chomel, B. B., Kasten, R. W., Floyd-Hawkins, K., Chi, B., Yamamoto, K., Roberts-Wilson, J., ... Koehler, J. E. (1996). Experimental transmission of Bartonella henselae by the cat flea. Journal of Clinical Microbiology, 34(8), 1952-1956.

Experimental transmission of Bartonella henselae by the cat flea. / Chomel, Bruno B; Kasten, Rickie W.; Floyd-Hawkins, Kim; Chi, Banghee; Yamamoto, Kazuhiro; Roberts-Wilson, Jill; Gurfield, A. Nikos; Abbott, Rachel C.; Pedersen, Niels C; Koehler, Jane E.

In: Journal of Clinical Microbiology, Vol. 34, No. 8, 08.1996, p. 1952-1956.

Research output: Contribution to journalArticle

Chomel, BB, Kasten, RW, Floyd-Hawkins, K, Chi, B, Yamamoto, K, Roberts-Wilson, J, Gurfield, AN, Abbott, RC, Pedersen, NC & Koehler, JE 1996, 'Experimental transmission of Bartonella henselae by the cat flea', Journal of Clinical Microbiology, vol. 34, no. 8, pp. 1952-1956.
Chomel BB, Kasten RW, Floyd-Hawkins K, Chi B, Yamamoto K, Roberts-Wilson J et al. Experimental transmission of Bartonella henselae by the cat flea. Journal of Clinical Microbiology. 1996 Aug;34(8):1952-1956.
Chomel, Bruno B ; Kasten, Rickie W. ; Floyd-Hawkins, Kim ; Chi, Banghee ; Yamamoto, Kazuhiro ; Roberts-Wilson, Jill ; Gurfield, A. Nikos ; Abbott, Rachel C. ; Pedersen, Niels C ; Koehler, Jane E. / Experimental transmission of Bartonella henselae by the cat flea. In: Journal of Clinical Microbiology. 1996 ; Vol. 34, No. 8. pp. 1952-1956.
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abstract = "Bartonella henselae is an emerging bacterial pathogen, causing cat scratch disease and bacillary angiomatosis. Cats bacteremic with B. henselae constitute a large reservoir from which humans become infected. Prevention of human infection depends on elucidation of the natural history and means of feline infection. We studied 47 cattery cats in a private home for 12 months to determine the longitudinal prevalence of B. henselae bacteremia, the prevalence of B. henselae in the fleas infesting these cats, and whether B. henselae is transmitted experimentally to cats via fleas. Vector-mediated transmission of B. henselae isolates was evaluated by removing fleas from the naturally bacteremic, flea-infested cattery cats and transferring these fleas to specific-pathogen-free (SPF) kittens housed in a controlled, arthropod- free University Animal Facility. B. henselae bacteremia was detected in 89{\%} of the 47 naturally infected cattery cats. A total of 132 fleas were removed from cats whose blood was simultaneously cultured during different seasons and were tested individually for the presence of B. henselae DNA by PCR. B. henselae DNA was detected in 34{\%} of 132 fleas, with seasonal variation, but without an association between the presence or the level of bacteremia in the corresponding cat. Cat fleas removed from bacteremic cattery cats transmitted B. henselae to five SPF kittens in two separate experiments; however, control SPF kittens ho used with highly bacteremic kittens in the absence of fleas did not become infected. These data demonstrate that the cat flea readily transmits B. henselae to cats. Control of feline infestation with this arthropod vector may provide an important strategy for the prevention of infection of both humans and cats.",
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