Experimental polymicrobial peritonitis-associated transcriptional regulation of murine endogenous retroviruses

Kiho Cho, Sophia Chiu, Young Kwan Lee, David G Greenhalgh, Jean Nemzek

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

Despite advancements in understanding the pathophysiology of sepsis, clinical outcomes are variable, and the mortality rate remains high among patients. We investigated whether expression of murine endogenous retroviruses (MuERVs), constituting ∼10% of the mouse genome, is differentially regulated in response to sepsis-elicited stress signals. ICR mice were subjected to cecal ligation and puncture, and MuERV expression was examined. There was evident regulation (induced or repressed) of MuERV expression in the liver and lung after cecal ligation and puncture. In particular, expression of several variant transcripts was increased, primarily in the liver, at 12 and/or 48 h: nine splicing variants and one 5.06-kb nonspliced transcript. Four novel splicing signals were also identified. Six variant transcripts were presumed to be splicing products of the 5.06-kb transcript, whereas the other three were envelope variants transcribed from at least five MuERV loci. These findings demonstrate that expression of certain MuERVs, including their envelope subgenomic transcripts, are altered during the course of sepsis pathogenesis.

Original languageEnglish (US)
Pages (from-to)147-158
Number of pages12
JournalShock
Volume32
Issue number2
DOIs
StatePublished - Aug 2009

Keywords

  • Cecal ligation and puncture
  • Endogenous retrovirus
  • Polymicrobial sepsis
  • Splicing variant
  • Transcription

ASJC Scopus subject areas

  • Critical Care and Intensive Care Medicine
  • Emergency Medicine

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