Abstract
Schwann cells were prepared from the sciatic nerves of newborn rats and cultured in a monolayer. Addition of lead acetate at concentrations between 0.4 and 10.0 μg/ml, levels comparable to those occurring in neural tissues and physiological fluids of lead-intoxicated rats, diminished both the baseline rate of proliferation of the Schwann cells and their response to the mitogens, axolemmal fragments, glial growth factor, and the adenosine 3':5'-cyclic monophosphate (cAMP) analogues 8-bromo-cAMP and dibutyryl-cAMP. This demonstrates a direct toxic effect of inorganic lead on Schwann cells. Lead acetate in this concentration range did not, however, inhibit the cAMP analogue-induced appearance of the 'myelin marker' lipid galactocerebroside on the surfaces of the cultured Schwann cells.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 462-468 |
| Number of pages | 7 |
| Journal | Annals of Neurology |
| Volume | 17 |
| Issue number | 5 |
| State | Published - 1985 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Neuroscience(all)
Cite this
Experimental lead neuropathy : Inorganic lead inhibits proliferation but not differentiation of Schwann cells. / Sobue, G.; Pleasure, David E.
In: Annals of Neurology, Vol. 17, No. 5, 1985, p. 462-468.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Experimental lead neuropathy
T2 - Inorganic lead inhibits proliferation but not differentiation of Schwann cells
AU - Sobue, G.
AU - Pleasure, David E
PY - 1985
Y1 - 1985
N2 - Schwann cells were prepared from the sciatic nerves of newborn rats and cultured in a monolayer. Addition of lead acetate at concentrations between 0.4 and 10.0 μg/ml, levels comparable to those occurring in neural tissues and physiological fluids of lead-intoxicated rats, diminished both the baseline rate of proliferation of the Schwann cells and their response to the mitogens, axolemmal fragments, glial growth factor, and the adenosine 3':5'-cyclic monophosphate (cAMP) analogues 8-bromo-cAMP and dibutyryl-cAMP. This demonstrates a direct toxic effect of inorganic lead on Schwann cells. Lead acetate in this concentration range did not, however, inhibit the cAMP analogue-induced appearance of the 'myelin marker' lipid galactocerebroside on the surfaces of the cultured Schwann cells.
AB - Schwann cells were prepared from the sciatic nerves of newborn rats and cultured in a monolayer. Addition of lead acetate at concentrations between 0.4 and 10.0 μg/ml, levels comparable to those occurring in neural tissues and physiological fluids of lead-intoxicated rats, diminished both the baseline rate of proliferation of the Schwann cells and their response to the mitogens, axolemmal fragments, glial growth factor, and the adenosine 3':5'-cyclic monophosphate (cAMP) analogues 8-bromo-cAMP and dibutyryl-cAMP. This demonstrates a direct toxic effect of inorganic lead on Schwann cells. Lead acetate in this concentration range did not, however, inhibit the cAMP analogue-induced appearance of the 'myelin marker' lipid galactocerebroside on the surfaces of the cultured Schwann cells.
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UR - http://www.scopus.com/inward/citedby.url?scp=0021836183&partnerID=8YFLogxK
M3 - Article
C2 - 2988413
AN - SCOPUS:0021836183
VL - 17
SP - 462
EP - 468
JO - Annals of Neurology
JF - Annals of Neurology
SN - 0364-5134
IS - 5
ER -