Experimental inoculation with human granulocytic ehrlichia agent derived from high- and low-passage cell culture in horses

Nicola Pusterla, John E Madigan, Kristin M. Asanovich, Joon Seok Chae, Elfriede Derock, Christian M. Leutenegger, Jeannine Berger Pusterla, Hans Lutz, J. Stephen Dumler

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

We report the successful infection throughout intravenous inoculation with low and high passage of in vitro-grown human granulocytic ehrlichiosis (HGE) agent in horses. Differences in disease severity but not in incubation time, hematological changes, PCR detection, ehrlichial load, seroconversion time, and titer range were noted between horses infected with a low and a high passage of in vitro-grown HGE agent.

Original languageEnglish (US)
Pages (from-to)1276-1278
Number of pages3
JournalJournal of Clinical Microbiology
Volume38
Issue number3
StatePublished - Mar 2000

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Ehrlichia
Ehrlichiosis
Horses
Cell Culture Techniques
Polymerase Chain Reaction
Infection
In Vitro Techniques

ASJC Scopus subject areas

  • Microbiology (medical)
  • Microbiology

Cite this

Experimental inoculation with human granulocytic ehrlichia agent derived from high- and low-passage cell culture in horses. / Pusterla, Nicola; Madigan, John E; Asanovich, Kristin M.; Chae, Joon Seok; Derock, Elfriede; Leutenegger, Christian M.; Pusterla, Jeannine Berger; Lutz, Hans; Dumler, J. Stephen.

In: Journal of Clinical Microbiology, Vol. 38, No. 3, 03.2000, p. 1276-1278.

Research output: Contribution to journalArticle

Pusterla, N, Madigan, JE, Asanovich, KM, Chae, JS, Derock, E, Leutenegger, CM, Pusterla, JB, Lutz, H & Dumler, JS 2000, 'Experimental inoculation with human granulocytic ehrlichia agent derived from high- and low-passage cell culture in horses', Journal of Clinical Microbiology, vol. 38, no. 3, pp. 1276-1278.
Pusterla, Nicola ; Madigan, John E ; Asanovich, Kristin M. ; Chae, Joon Seok ; Derock, Elfriede ; Leutenegger, Christian M. ; Pusterla, Jeannine Berger ; Lutz, Hans ; Dumler, J. Stephen. / Experimental inoculation with human granulocytic ehrlichia agent derived from high- and low-passage cell culture in horses. In: Journal of Clinical Microbiology. 2000 ; Vol. 38, No. 3. pp. 1276-1278.
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