Experimental infection of Peromyscus californicus with Toxoplasma gondii

Haydee A. Dabritz; Melissa A. Miller; Andrea E. Packham; Daniel Rejmanek; Christian M. Leutenegger; Ian Gardner; Edward R Atwill; Patricia A Conrad

doi:10.1645/GE-1210.1

Experimental infection of Peromyscus californicus with Toxoplasma gondii

Haydee A. Dabritz, Melissa A. Miller, Andrea E. Packham, Daniel Rejmanek, Christian M. Leutenegger, Ian Gardner, Edward R Atwill, Patricia A Conrad

Research output: Contribution to journal › Article

5 Scopus citations

Abstract

Eight female Peromyscus californicus were infected with 10² or 10⁴ Toxoplasma gondii culture-derived tachyzoites (Type II or X) isolated from southern sea otters. All but 2 mice survived infection and developed antibodies to T. gondii. The 2 fatally infected mice were inoculated with 10⁴ tachyzoites of the Type X strain. Parasite detection by immunohistochemistry (IHC) and DNA amplification with 2 polymerase chain reaction (PCR) methods was compared for brain, heart, lung, liver, spleen, biceps muscle, and tongue, at a mean of 41 days postinfection. Parasites were detected most commonly by IHC in spleen (8/8) and brain (6/8). DNA amplification by PCR was most successful from brain, heart, and spleen.

Original language	English (US)
Pages (from-to)	1360-1364
Number of pages	5
Journal	Journal of Parasitology
Volume	93
Issue number	6
DOIs	https://doi.org/10.1645/GE-1210.1
State	Published - Dec 2007

ASJC Scopus subject areas

Agricultural and Biological Sciences(all)
Parasitology
Microbiology

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Dabritz, H. A., Miller, M. A., Packham, A. E., Rejmanek, D., Leutenegger, C. M., Gardner, I., Atwill, E. R., & Conrad, P. A. (2007). Experimental infection of Peromyscus californicus with Toxoplasma gondii. Journal of Parasitology, 93(6), 1360-1364. https://doi.org/10.1645/GE-1210.1

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abstract = "Eight female Peromyscus californicus were infected with 102 or 104 Toxoplasma gondii culture-derived tachyzoites (Type II or X) isolated from southern sea otters. All but 2 mice survived infection and developed antibodies to T. gondii. The 2 fatally infected mice were inoculated with 104 tachyzoites of the Type X strain. Parasite detection by immunohistochemistry (IHC) and DNA amplification with 2 polymerase chain reaction (PCR) methods was compared for brain, heart, lung, liver, spleen, biceps muscle, and tongue, at a mean of 41 days postinfection. Parasites were detected most commonly by IHC in spleen (8/8) and brain (6/8). DNA amplification by PCR was most successful from brain, heart, and spleen.",

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AU - Atwill, Edward R

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