Experimental infection of Peromyscus californicus with Toxoplasma gondii

Haydee A. Dabritz, Melissa A. Miller, Andrea E. Packham, Daniel Rejmanek, Christian M. Leutenegger, Ian Gardner, Edward R Atwill, Patricia A Conrad

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

Eight female Peromyscus californicus were infected with 102 or 104 Toxoplasma gondii culture-derived tachyzoites (Type II or X) isolated from southern sea otters. All but 2 mice survived infection and developed antibodies to T. gondii. The 2 fatally infected mice were inoculated with 104 tachyzoites of the Type X strain. Parasite detection by immunohistochemistry (IHC) and DNA amplification with 2 polymerase chain reaction (PCR) methods was compared for brain, heart, lung, liver, spleen, biceps muscle, and tongue, at a mean of 41 days postinfection. Parasites were detected most commonly by IHC in spleen (8/8) and brain (6/8). DNA amplification by PCR was most successful from brain, heart, and spleen.

Original languageEnglish (US)
Pages (from-to)1360-1364
Number of pages5
JournalJournal of Parasitology
Volume93
Issue number6
DOIs
StatePublished - Dec 2007

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Parasitology
  • Microbiology

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