Experimental infection of Peromyscus californicus with Toxoplasma gondii

Haydee A. Dabritz; Melissa A. Miller; Andrea E. Packham; Daniel Rejmanek; Christian M. Leutenegger; Ian Gardner; Edward R Atwill; Patricia A Conrad

doi:10.1645/GE-1210.1

Experimental infection of Peromyscus californicus with Toxoplasma gondii

Haydee A. Dabritz, Melissa A. Miller, Andrea E. Packham, Daniel Rejmanek, Christian M. Leutenegger, Ian Gardner, Edward R Atwill, Patricia A Conrad

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5 Scopus citations

Abstract

Eight female Peromyscus californicus were infected with 10² or 10⁴ Toxoplasma gondii culture-derived tachyzoites (Type II or X) isolated from southern sea otters. All but 2 mice survived infection and developed antibodies to T. gondii. The 2 fatally infected mice were inoculated with 10⁴ tachyzoites of the Type X strain. Parasite detection by immunohistochemistry (IHC) and DNA amplification with 2 polymerase chain reaction (PCR) methods was compared for brain, heart, lung, liver, spleen, biceps muscle, and tongue, at a mean of 41 days postinfection. Parasites were detected most commonly by IHC in spleen (8/8) and brain (6/8). DNA amplification by PCR was most successful from brain, heart, and spleen.

Original language	English (US)
Pages (from-to)	1360-1364
Number of pages	5
Journal	Journal of Parasitology
Volume	93
Issue number	6
DOIs	https://doi.org/10.1645/GE-1210.1
State	Published - Dec 2007

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ASJC Scopus subject areas

Agricultural and Biological Sciences(all)
Parasitology
Microbiology

Cite this

Dabritz, H. A., Miller, M. A., Packham, A. E., Rejmanek, D., Leutenegger, C. M., Gardner, I., Atwill, E. R., & Conrad, P. A. (2007). Experimental infection of Peromyscus californicus with Toxoplasma gondii. Journal of Parasitology, 93(6), 1360-1364. https://doi.org/10.1645/GE-1210.1

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abstract = "Eight female Peromyscus californicus were infected with 102 or 104 Toxoplasma gondii culture-derived tachyzoites (Type II or X) isolated from southern sea otters. All but 2 mice survived infection and developed antibodies to T. gondii. The 2 fatally infected mice were inoculated with 104 tachyzoites of the Type X strain. Parasite detection by immunohistochemistry (IHC) and DNA amplification with 2 polymerase chain reaction (PCR) methods was compared for brain, heart, lung, liver, spleen, biceps muscle, and tongue, at a mean of 41 days postinfection. Parasites were detected most commonly by IHC in spleen (8/8) and brain (6/8). DNA amplification by PCR was most successful from brain, heart, and spleen.",

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AU - Atwill, Edward R

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10.1645/GE-1210.1