Abstract
Eight female Peromyscus californicus were infected with 102 or 104 Toxoplasma gondii culture-derived tachyzoites (Type II or X) isolated from southern sea otters. All but 2 mice survived infection and developed antibodies to T. gondii. The 2 fatally infected mice were inoculated with 104 tachyzoites of the Type X strain. Parasite detection by immunohistochemistry (IHC) and DNA amplification with 2 polymerase chain reaction (PCR) methods was compared for brain, heart, lung, liver, spleen, biceps muscle, and tongue, at a mean of 41 days postinfection. Parasites were detected most commonly by IHC in spleen (8/8) and brain (6/8). DNA amplification by PCR was most successful from brain, heart, and spleen.
Original language | English (US) |
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Pages (from-to) | 1360-1364 |
Number of pages | 5 |
Journal | Journal of Parasitology |
Volume | 93 |
Issue number | 6 |
DOIs | |
State | Published - Dec 2007 |
ASJC Scopus subject areas
- Agricultural and Biological Sciences(all)
- Parasitology
- Microbiology