Experimental infection of domestic cats with passaged genotype I Bartonella henselae

Jonathan A. Werner, Rickie W. Kasten, Sunlian Feng, Jane E Sykes, Emir Hodzic, Michelle R. Salemi, Stephen W Barthold, Bruno B Chomel

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The influence of in vitro passage on Bartonella henselae pathogenesis in cats has not been thoroughly evaluated. Our objective was to examine the bacterial kinetics and humoral immune responses in cats experimentally infected with three different in vitro passages of B. henselae F1, a genotype I strain of feline origin. The F1 strain was in vitro passaged 20 and 40 times, and each was inoculated into a group of 5 cats. The kinetics of bacteremia and the feline humoral immune response to bacterial antigens were compared to a previous study involving a group of six cats inoculated with the original F1 strain. Among the three groups of cats, the kinetics of bacteremia profiles and the humoral immune responses to B. henselae lysates were similar. The influence of passage on bacterial membrane proteins was examined. In vitro passage altered the expression of 4/17 (23.5%) bacterial membrane proteins and 6/15 (40%) bacterial membrane antigens. An association between poor seroreactivity to three lysate antigens (15-, 18- and 45 kDa), prolonged bacteremia and decreased serum bactericidal activity was noted. Our data show that in vitro passage of B. henselae did not alter the kinetics of bacteremia, including the occurrence of relapsing bacteremia, in experimentally infected cats. This suggests that highly passaged strains may not be suitable for future vaccination studies. Furthermore, in vitro passage results in phenotypic and antigenic changes in the bacterial membrane protein profile, which warrants caution in the interpretation of studies involving passaged B. henselae strains.

Original languageEnglish (US)
Pages (from-to)290-297
Number of pages8
JournalVeterinary Microbiology
Volume122
Issue number3-4
DOIs
StatePublished - Jun 21 2007

Fingerprint

Bartonella henselae
Bacteremia
Cats
bacteremia
Genotype
cats
Bacterial Proteins
genotype
Humoral Immunity
Infection
infection
bacterial proteins
Bacterial Antigens
Membrane Proteins
Felidae
humoral immunity
membrane proteins
kinetics
bacterial antigens
antigens

Keywords

  • Bartonella henselae
  • Feline
  • Vaccine

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Microbiology
  • veterinary(all)

Cite this

Experimental infection of domestic cats with passaged genotype I Bartonella henselae. / Werner, Jonathan A.; Kasten, Rickie W.; Feng, Sunlian; Sykes, Jane E; Hodzic, Emir; Salemi, Michelle R.; Barthold, Stephen W; Chomel, Bruno B.

In: Veterinary Microbiology, Vol. 122, No. 3-4, 21.06.2007, p. 290-297.

Research output: Contribution to journalArticle

Werner, Jonathan A. ; Kasten, Rickie W. ; Feng, Sunlian ; Sykes, Jane E ; Hodzic, Emir ; Salemi, Michelle R. ; Barthold, Stephen W ; Chomel, Bruno B. / Experimental infection of domestic cats with passaged genotype I Bartonella henselae. In: Veterinary Microbiology. 2007 ; Vol. 122, No. 3-4. pp. 290-297.
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