Experimental cross-infections with Ehrlichia phagocytophila and human granulocytic ehrlichia-like agent in cows and horses

Nicola Pusterla, J. B. Pusterla, U. Braun, H. Lutz

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Four cows and four horses were infected experimentally with Ehrlichia phagocytophila, the cause of tickborne fever in ruminants, and with human granulocytic ehrlichia-like agent, a recently discovered species that infects people, horses and dogs in the USA and Europe. They were infected in either order, 30 days apart, to investigate serological cross-reactivity within the E phagocytophila genogroup. The course of infection was assessed by routine clinical, haematological, serological and polymerase chain reaction (PCR) examinations. Two of the cows infected with Ephagocytophila and two of the horses infected with granulocytic ehrlichia-like agent, developed characteristic signs of ehrlichiosis. When the same animals were infected with their heterologous ehrlichial isolate 30 days later, they did not develop clinical signs of disease. The infection of the other two cows with human granulocytic ehrlichia-like agent and the other two horses with E phagocytophila, resulted in asymptomatic seroconversion. When the same animals were infected with their homologous ehrlichial isolate 30 days later, they remained asymptomatic and had normal haematological results and negative PCRS until the end of the monitoring period, 60 days after the first infection. In these animals, there was an increase in antibody titre after the second infection which was interpreted as a specific immune response, and as a reactivation of the immune response to the first infection.

Original languageEnglish (US)
Pages (from-to)311-314
Number of pages4
JournalVeterinary Record
Volume145
Issue number11
StatePublished - Sep 11 1999
Externally publishedYes

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Ehrlichia
Anaplasma phagocytophilum
cross infection
Cross Infection
Horses
horses
cows
Infection
infection
immune response
Ehrlichiosis
ehrlichiosis
animals
Ruminants
seroconversion
cross reaction
fever
ruminants
Fever
polymerase chain reaction

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Experimental cross-infections with Ehrlichia phagocytophila and human granulocytic ehrlichia-like agent in cows and horses. / Pusterla, Nicola; Pusterla, J. B.; Braun, U.; Lutz, H.

In: Veterinary Record, Vol. 145, No. 11, 11.09.1999, p. 311-314.

Research output: Contribution to journalArticle

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