Experimental bluetongue virus infection of sheep; effect of previous vaccination: clinical and immunologic studies.

C. R. Mahrt, Bennie Osburn

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Clinical and immunologic responses of sheep to vaccination and subsequent bluetongue virus (BTV) challenge exposure were studied and compared with those of non-vaccinated sheep. Sheep were vaccinated with inactivated BTV administered with aluminum hydroxide and cimetidine or levamisole. After sheep were vaccinated, precipitating group-specific antibodies to BTV were detected, but serotype-specific neutralizing antibodies were not detected. Cellular immune responses (lymphocyte blastogenesis) to BTV were not detected. After virulent BTV challenge exposure, vaccinated and nonvaccinated sheep developed acute clinical disease of similar severity. Clinical signs included hyperemia and petechiae of oral mucosa and coronary bands of the feet, excess salivation, nasal discharge with crusting, ulceration of the muzzle, and edema of lips and intermandibular space. Marked increases in serum creatine kinase activity were associated with stiff gait, reluctance to move, and vomiting. Fever and leukopenia were detected in most of the challenge-exposed sheep. Viremia and neutralizing antibodies were detected in vaccinated and nonvaccinated sheep after challenge exposure. Bluetongue virus-specific reaginic antibodies were not detected in sera from any of the sheep when the passive cutaneous anaphylaxis test was used.

Original languageEnglish (US)
Pages (from-to)1191-1197
Number of pages7
JournalAmerican Journal of Veterinary Research
Volume47
Issue number6
StatePublished - Jun 1 1986
Externally publishedYes

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Bluetongue virus
Virus Diseases
Sheep
Vaccination
vaccination
sheep
infection
Neutralizing Antibodies
neutralizing antibodies
digestive tract mucosa
cimetidine
Salivation
Aluminum Hydroxide
anaphylaxis
aluminum hydroxide
Levamisole
salivation
leukopenia
Passive Cutaneous Anaphylaxis
antibodies

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Experimental bluetongue virus infection of sheep; effect of previous vaccination : clinical and immunologic studies. / Mahrt, C. R.; Osburn, Bennie.

In: American Journal of Veterinary Research, Vol. 47, No. 6, 01.06.1986, p. 1191-1197.

Research output: Contribution to journalArticle

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