Experimental and computational laser tissue welding using a protein patch

Ward Small IV, Nicholas J. Heredia, Duncan J. Maitland, David C. Eder, Peter M. Celliers, Luiz B. Da Silva, Richard A. London, Dennis L Matthews

Research output: Contribution to journalArticlepeer-review

6 Scopus citations


An in vitro study of laser tissue welding mediated with a dye-enhanced protein patch was conducted. Fresh sections of porcine aorta were used for the experiments. Arteriotomies were treated using an indocyanine green dye-enhanced collagen patch activated by an 805-nm continuous-wave fiber-delivered diode laser. Temperature histories of the surface of the weld site were obtained using a hollow glass optical fiber-based two-color infrared thermometer. The experimental effort was complemented by simulations with the LATIS (LAser-TISsue) computer code, which uses coupled Monte Carlo, thermal transport, and mass transport models. Comparison of simulated and experimental thermal data indicated that evaporative cooling clamped the surface temperature of the weld site below 100 °C. For fluences of approximately 200 J/cm2, peak surface temperatures averaged 74°C and acute burst strengths consistently exceeded 0.14×106 dyn/cm (hoop tension). The combination of experimental and simulation results showed that the inclusion of water transport and evaporative losses in the computer code has a significant impact on the thermal distributions and hydration levels throughout the tissue volume. The solid-matrix protein patch provided a means of controllable energy delivery and yielded consistently strong welds.

Original languageEnglish (US)
Pages (from-to)96-101
Number of pages6
JournalJournal of Biomedical Optics
Issue number1
StatePublished - 1998
Externally publishedYes


  • Collagen
  • Infrared thermometry
  • Modeling
  • Surface temperature
  • Tissue fusion

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Radiology Nuclear Medicine and imaging
  • Atomic and Molecular Physics, and Optics
  • Radiological and Ultrasound Technology


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