Experiences with infectious cDNA clones of equine arteritis virus: Lessons learned and insights gained

Udeni B R Balasuriya, Jianqiang Zhang, Yun Young Go, Nigel J Maclachlan

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

The advent of recombinant DNA technology, development of infectious cDNA clones of RNA viruses, and reverse genetic technologies have revolutionized how viruses are studied. Genetic manipulation of full-length cDNA clones has become an especially important and widely used tool to study the biology, pathogenesis, and virulence determinants of both positive and negative stranded RNA viruses. The first full-length infectious cDNA clone of equine arteritis virus (EAV) was developed in 1996 and was also the first full-length infectious cDNA clone constructed from a member of the order Nidovirales. This clone was extensively used to characterize the molecular biology of EAV and other Nidoviruses. The objective of this review is to summarize the characterization of the virulence (or attenuation) phenotype of the recombinant viruses derived from several infectious cDNA clones of EAV in horses, as well as their application for characterization of the molecular basis of viral neutralization, persistence, and cellular tropism.

Original languageEnglish (US)
Pages (from-to)388-403
Number of pages16
JournalVirology
Volume462-463
Issue number1
DOIs
StatePublished - 2014

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Keywords

  • Arteriviruses
  • CDNA clones
  • EAV
  • Equine arteritis virus
  • Equine viral arteritis
  • EVA
  • Reverse genetics

ASJC Scopus subject areas

  • Virology

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