Experience-dependent neuroplasticity of the developing hypothalamus: integrative epigenomic approaches

Annie Vogel Ciernia, Benjamin I. Laufer, Keith W. Dunaway, Charles E. Mordaunt, Rochelle L. Coulson, Theresa S. Totah, Danielle S. Stolzenberg, Jaime C. Frahm, Akanksha Singh-Taylor, Tallie Z. Baram, Janine M LaSalle, Dag H. Yasui

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Augmented maternal care during the first postnatal week promotes life-long stress resilience and improved memory compared with the outcome of routine rearing conditions. Recent evidence suggests that this programming commences with altered synaptic connectivity of stress sensitive hypothalamic neurons. However, the epigenomic basis of the long-lived consequences is not well understood. Here, we employed whole-genome bisulfite sequencing (WGBS), RNA-sequencing (RNA-seq), and a multiplex microRNA (miRNA) assay to examine the effects of augmented maternal care on DNA cytosine methylation, gene expression, and miRNA expression. A total of 9,439 differentially methylated regions (DMRs) associated with augmented maternal care were identified in male offspring hypothalamus, as well as a modest but significant decrease in global DNA methylation. Differentially methylated and expressed genes were enriched for functions in neurotransmission, neurodevelopment, protein synthesis, and oxidative phosphorylation, as well as known stress response genes. Twenty prioritized genes were identified as highly relevant to the stress resiliency phenotype. This combined unbiased approach enabled the discovery of novel genes and gene pathways that advance our understanding of the epigenomic mechanisms underlying the effects of maternal care on the developing brain.

Original languageEnglish (US)
Pages (from-to)1-13
Number of pages13
JournalEpigenetics
DOIs
StateAccepted/In press - May 1 2018

Fingerprint

Neuronal Plasticity
Epigenomics
Hypothalamus
Mothers
DNA Methylation
MicroRNAs
Genes
RNA Sequence Analysis
Cytosine
Oxidative Phosphorylation
Genetic Association Studies
Psychological Stress
Synaptic Transmission
Genome
RNA
Phenotype
Gene Expression
Neurons
Brain
Proteins

Keywords

  • Augmented maternal care
  • DNA methylation
  • early-life stress
  • epigenomics
  • hypothalamus
  • resilience

ASJC Scopus subject areas

  • Molecular Biology
  • Cancer Research

Cite this

Vogel Ciernia, A., Laufer, B. I., Dunaway, K. W., Mordaunt, C. E., Coulson, R. L., Totah, T. S., ... Yasui, D. H. (Accepted/In press). Experience-dependent neuroplasticity of the developing hypothalamus: integrative epigenomic approaches. Epigenetics, 1-13. https://doi.org/10.1080/15592294.2018.1451720

Experience-dependent neuroplasticity of the developing hypothalamus : integrative epigenomic approaches. / Vogel Ciernia, Annie; Laufer, Benjamin I.; Dunaway, Keith W.; Mordaunt, Charles E.; Coulson, Rochelle L.; Totah, Theresa S.; Stolzenberg, Danielle S.; Frahm, Jaime C.; Singh-Taylor, Akanksha; Baram, Tallie Z.; LaSalle, Janine M; Yasui, Dag H.

In: Epigenetics, 01.05.2018, p. 1-13.

Research output: Contribution to journalArticle

Vogel Ciernia, A, Laufer, BI, Dunaway, KW, Mordaunt, CE, Coulson, RL, Totah, TS, Stolzenberg, DS, Frahm, JC, Singh-Taylor, A, Baram, TZ, LaSalle, JM & Yasui, DH 2018, 'Experience-dependent neuroplasticity of the developing hypothalamus: integrative epigenomic approaches', Epigenetics, pp. 1-13. https://doi.org/10.1080/15592294.2018.1451720
Vogel Ciernia, Annie ; Laufer, Benjamin I. ; Dunaway, Keith W. ; Mordaunt, Charles E. ; Coulson, Rochelle L. ; Totah, Theresa S. ; Stolzenberg, Danielle S. ; Frahm, Jaime C. ; Singh-Taylor, Akanksha ; Baram, Tallie Z. ; LaSalle, Janine M ; Yasui, Dag H. / Experience-dependent neuroplasticity of the developing hypothalamus : integrative epigenomic approaches. In: Epigenetics. 2018 ; pp. 1-13.
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