Exosomes and HIV Gag bud from endosome-like domains of the T cell plasma membrane

Amy M. Booth, Yi Fang, Jonathan K. Fallon, Jr Ming Yang, James Hildreth, Stephen J. Gould, S. Sandefur, V. Varthakavi

Research output: Contribution to journalArticle

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Abstract

Exosomes are secreted, single membrane organelles of ∼100 nm diameter. Their biogenesis is typically thought to occur in a two-step process involving (1) outward vesicle budding at limiting membranes of endosomes (outward = away from the cytoplasm), which generates intralumenal vesicles, followed by (2) endosome-plasma membrane fusion, which releases these internal vesicles into the extracellular milieu as exosomes. In this study, we present evidence that certain cells, including Jurkat T cells, possess discrete domains of plasma membrane that are enriched for exosomal and endosomal proteins, retain the endosomal property of outward vesicle budding, and serve as sites of immediate exosome biogenesis. It has been hypothesized that retroviruses utilize the exosome biogenesis pathway for the formation of infectious particles. In support of this, we find that Jurkat T cells direct the key budding factor of HIV, HIV Gag, to these endosome-like domains of plasma membrane and secrete HIV Gag from the cell in exosomes.

Original languageEnglish (US)
Pages (from-to)923-935
Number of pages13
JournalJournal of Cell Biology
Volume172
Issue number6
DOIs
StatePublished - Mar 13 2006
Externally publishedYes

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ASJC Scopus subject areas

  • Cell Biology

Cite this

Booth, A. M., Fang, Y., Fallon, J. K., Yang, J. M., Hildreth, J., Gould, S. J., Sandefur, S., & Varthakavi, V. (2006). Exosomes and HIV Gag bud from endosome-like domains of the T cell plasma membrane. Journal of Cell Biology, 172(6), 923-935. https://doi.org/10.1083/jcb.200508014