Exercise induces an increase in muscle UCP3 as a component of the increase in mitochondrial biogenesis

Terry E. Jones, Keith Baar, Edward Ojuka, May Chen, John O. Holloszy

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

Previous studies have indicated that exercise acutely induces large increases in uncoupling protein-3 (UCP3) in skeletal muscle, whereas endurance training results in marked decreases in muscle UCP3. Because UCP3 expression appears to be regulated by the same mechanism as other mitochondrial constituents, it seemed unlikely that exercise would result in such large and divergent changes in mitochondrial composition. The purpose of this study was to test the hypothesis that major changes in UCP3 protein concentration do not occur independently of mitochondrial biogenesis and that UCP3 increases as a component of the exercise-induced increase in mitochondria. We found a large increase in UCP3 mRNA immediately and 3 h after a bout of swimming. UCP3 protein concentration was increased ∼35% 18 h after a single exercise bout, ∼63% after 3 days, and ∼84% after 10 days of exercise. These increases in UCP3 roughly paralleled those of other mitochondrial marker proteins. Our results are consistent with the interpretation that endurance exercise induces an adaptive increase in mitochondria that have a normal content of UCP3.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Endocrinology and Metabolism
Volume284
Issue number1 47-1
StatePublished - Jan 1 2003
Externally publishedYes

Fingerprint

Muscle Proteins
Organelle Biogenesis
Muscle
Proteins
Mitochondria
Uncoupling Protein 3
Durability
Mitochondrial Proteins
Skeletal Muscle
Messenger RNA

Keywords

  • Cytochrome c
  • Cytochrome oxidase
  • Gene expression
  • Skeletal muscle

ASJC Scopus subject areas

  • Physiology
  • Endocrinology
  • Biochemistry

Cite this

Exercise induces an increase in muscle UCP3 as a component of the increase in mitochondrial biogenesis. / Jones, Terry E.; Baar, Keith; Ojuka, Edward; Chen, May; Holloszy, John O.

In: American Journal of Physiology - Endocrinology and Metabolism, Vol. 284, No. 1 47-1, 01.01.2003.

Research output: Contribution to journalArticle

Jones, Terry E. ; Baar, Keith ; Ojuka, Edward ; Chen, May ; Holloszy, John O. / Exercise induces an increase in muscle UCP3 as a component of the increase in mitochondrial biogenesis. In: American Journal of Physiology - Endocrinology and Metabolism. 2003 ; Vol. 284, No. 1 47-1.
@article{165c0159c3a14a339a631ac457952cbf,
title = "Exercise induces an increase in muscle UCP3 as a component of the increase in mitochondrial biogenesis",
abstract = "Previous studies have indicated that exercise acutely induces large increases in uncoupling protein-3 (UCP3) in skeletal muscle, whereas endurance training results in marked decreases in muscle UCP3. Because UCP3 expression appears to be regulated by the same mechanism as other mitochondrial constituents, it seemed unlikely that exercise would result in such large and divergent changes in mitochondrial composition. The purpose of this study was to test the hypothesis that major changes in UCP3 protein concentration do not occur independently of mitochondrial biogenesis and that UCP3 increases as a component of the exercise-induced increase in mitochondria. We found a large increase in UCP3 mRNA immediately and 3 h after a bout of swimming. UCP3 protein concentration was increased ∼35{\%} 18 h after a single exercise bout, ∼63{\%} after 3 days, and ∼84{\%} after 10 days of exercise. These increases in UCP3 roughly paralleled those of other mitochondrial marker proteins. Our results are consistent with the interpretation that endurance exercise induces an adaptive increase in mitochondria that have a normal content of UCP3.",
keywords = "Cytochrome c, Cytochrome oxidase, Gene expression, Skeletal muscle",
author = "Jones, {Terry E.} and Keith Baar and Edward Ojuka and May Chen and Holloszy, {John O.}",
year = "2003",
month = "1",
day = "1",
language = "English (US)",
volume = "284",
journal = "American Journal of Physiology - Renal Fluid and Electrolyte Physiology",
issn = "1931-857X",
publisher = "American Physiological Society",
number = "1 47-1",

}

TY - JOUR

T1 - Exercise induces an increase in muscle UCP3 as a component of the increase in mitochondrial biogenesis

AU - Jones, Terry E.

AU - Baar, Keith

AU - Ojuka, Edward

AU - Chen, May

AU - Holloszy, John O.

PY - 2003/1/1

Y1 - 2003/1/1

N2 - Previous studies have indicated that exercise acutely induces large increases in uncoupling protein-3 (UCP3) in skeletal muscle, whereas endurance training results in marked decreases in muscle UCP3. Because UCP3 expression appears to be regulated by the same mechanism as other mitochondrial constituents, it seemed unlikely that exercise would result in such large and divergent changes in mitochondrial composition. The purpose of this study was to test the hypothesis that major changes in UCP3 protein concentration do not occur independently of mitochondrial biogenesis and that UCP3 increases as a component of the exercise-induced increase in mitochondria. We found a large increase in UCP3 mRNA immediately and 3 h after a bout of swimming. UCP3 protein concentration was increased ∼35% 18 h after a single exercise bout, ∼63% after 3 days, and ∼84% after 10 days of exercise. These increases in UCP3 roughly paralleled those of other mitochondrial marker proteins. Our results are consistent with the interpretation that endurance exercise induces an adaptive increase in mitochondria that have a normal content of UCP3.

AB - Previous studies have indicated that exercise acutely induces large increases in uncoupling protein-3 (UCP3) in skeletal muscle, whereas endurance training results in marked decreases in muscle UCP3. Because UCP3 expression appears to be regulated by the same mechanism as other mitochondrial constituents, it seemed unlikely that exercise would result in such large and divergent changes in mitochondrial composition. The purpose of this study was to test the hypothesis that major changes in UCP3 protein concentration do not occur independently of mitochondrial biogenesis and that UCP3 increases as a component of the exercise-induced increase in mitochondria. We found a large increase in UCP3 mRNA immediately and 3 h after a bout of swimming. UCP3 protein concentration was increased ∼35% 18 h after a single exercise bout, ∼63% after 3 days, and ∼84% after 10 days of exercise. These increases in UCP3 roughly paralleled those of other mitochondrial marker proteins. Our results are consistent with the interpretation that endurance exercise induces an adaptive increase in mitochondria that have a normal content of UCP3.

KW - Cytochrome c

KW - Cytochrome oxidase

KW - Gene expression

KW - Skeletal muscle

UR - http://www.scopus.com/inward/record.url?scp=0037227527&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0037227527&partnerID=8YFLogxK

M3 - Article

C2 - 12388129

AN - SCOPUS:0037227527

VL - 284

JO - American Journal of Physiology - Renal Fluid and Electrolyte Physiology

JF - American Journal of Physiology - Renal Fluid and Electrolyte Physiology

SN - 1931-857X

IS - 1 47-1

ER -