Excretory, Secretory, and Tissue Residues after Label and Extra-label Administration of Flunixin Meglumine to Saline- or Lipopolysaccharide-Exposed Dairy Cows

David J. Smith, Weilin L. Shelver, Ronald E. Baynes, Lisa A Tell, Ronette Gehring, Mengjie Li, Terry Dutko, J. W. Schroeder, Grant Herges, Jim E. Riviere

Research output: Contribution to journalArticle

15 Scopus citations


Twenty lactating dairy cattle were intravenously infused with either lipopolysaccharide (LPS) (n = 10) or sterile saline (n = 10). Five cattle in each group received three doses of flunixin meglumine administered by either intravenous infusion or intramuscular injection at 24 h intervals. Milk, urine, and tissues were collected. Thirty-six hours after the last flunixin administration, milk from six cows contained 5-hydroxyflunixin (5OHF) levels greater than the milk tolerance of 2 ng/mL; by 48 h, milk from two cows, a saline and a LPS-treated animal, had violative milk concentrations of 5OHF. A single animal treated with LPS and intramuscular flunixin contained violative flunixin residues in liver. The ratio of urinary flunixin/5OHF was correlated (P < 0.01; R<sup>2</sup> = 0.946) with liver flunixin residues in LPS-treated animals, but not (P = 0.96; R<sup>2</sup> = 0.003) in cows treated with saline in lieu of LPS. Violative residues of flunixin in dairy cattle may be related to LPS inhibition of flunixin metabolism.

Original languageEnglish (US)
Pages (from-to)4893-4901
Number of pages9
JournalJournal of Agricultural and Food Chemistry
Issue number19
StatePublished - May 20 2015



  • dairy
  • extra-label
  • flunixin
  • holstein
  • liver
  • metabolite ratio
  • milk
  • residue

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Chemistry(all)

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