Excitotoxic superoxide production and neuronal death require both ionotropic and non-ionotropic NMDA receptor signaling

Angela M. Minnella, Jerry X. Zhao, Xiangning Jiang, Emil Jakobsen, Fuxin Lu, Long Wu, Jamel El-Benna, John Gray, Raymond A. Swanson

Research output: Contribution to journalArticle

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Abstract

NMDA-type glutamate receptors (NMDAR) trigger superoxide production by neuronal NADPH oxidase-2 (NOX2), which if sustained leads to cell death. This process involves Ca 2+ influx through NMDAR channels. By contrast, comparable Ca 2+ influx by other routes does not induce NOX2 activation or cell death. This contrast has been attributed to site-specific effects of Ca 2+ flux through NMDAR. Here we show instead that it stems from non-ionotropic signaling by NMDAR GluN2B subunits. To evaluate non-ionotropic effects, mouse cortical neurons were treated with NMDA together with 7-chlorokynurenate, L-689,560, or MK-801, which block Ca 2+ influx through NMDAR channels but not NMDA binding. NMDA-induced superoxide formation was prevented by the channel blockers, restored by concurrent Ca 2+ influx through ionomycin or voltage-gated calcium channels, and not induced by the Ca 2+ influx in the absence of NMDAR ligand binding. Neurons expressing either GluN2B subunits or chimeric GluN2A/GluN2B C-terminus subunits exhibited NMDA-induced superoxide production, whereas neurons expressing chimeric GluN2B/GluN2A C-terminus subunits did not. Neuronal NOX2 activation requires phosphoinositide 3-kinase (PI3K), and NMDA binding to NMDAR increased PI3K association with NMDA GluN2B subunits independent of Ca 2+ influx. These findings identify a non-ionotropic signaling pathway that links NMDAR to NOX2 activation through the C-terminus domain of GluN2B.

Original languageEnglish (US)
Article number17522
JournalScientific Reports
Volume8
Issue number1
DOIs
StatePublished - Dec 1 2018

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Glutamate Receptors
N-Methyl-D-Aspartate Receptors
Superoxides
N-Methylaspartate
NADPH Oxidase
1-Phosphatidylinositol 4-Kinase
Neurons
4-trans-2-carboxy-5,7-dichloro-4-phenylaminocarbonylamino-1,2,3,4-tetrahydroquinoline
Cell Death
Ionomycin
Dizocilpine Maleate
Calcium Channels
Ligands

ASJC Scopus subject areas

  • General

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Excitotoxic superoxide production and neuronal death require both ionotropic and non-ionotropic NMDA receptor signaling. / Minnella, Angela M.; Zhao, Jerry X.; Jiang, Xiangning; Jakobsen, Emil; Lu, Fuxin; Wu, Long; El-Benna, Jamel; Gray, John; Swanson, Raymond A.

In: Scientific Reports, Vol. 8, No. 1, 17522, 01.12.2018.

Research output: Contribution to journalArticle

Minnella, Angela M. ; Zhao, Jerry X. ; Jiang, Xiangning ; Jakobsen, Emil ; Lu, Fuxin ; Wu, Long ; El-Benna, Jamel ; Gray, John ; Swanson, Raymond A. / Excitotoxic superoxide production and neuronal death require both ionotropic and non-ionotropic NMDA receptor signaling. In: Scientific Reports. 2018 ; Vol. 8, No. 1.
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abstract = "NMDA-type glutamate receptors (NMDAR) trigger superoxide production by neuronal NADPH oxidase-2 (NOX2), which if sustained leads to cell death. This process involves Ca 2+ influx through NMDAR channels. By contrast, comparable Ca 2+ influx by other routes does not induce NOX2 activation or cell death. This contrast has been attributed to site-specific effects of Ca 2+ flux through NMDAR. Here we show instead that it stems from non-ionotropic signaling by NMDAR GluN2B subunits. To evaluate non-ionotropic effects, mouse cortical neurons were treated with NMDA together with 7-chlorokynurenate, L-689,560, or MK-801, which block Ca 2+ influx through NMDAR channels but not NMDA binding. NMDA-induced superoxide formation was prevented by the channel blockers, restored by concurrent Ca 2+ influx through ionomycin or voltage-gated calcium channels, and not induced by the Ca 2+ influx in the absence of NMDAR ligand binding. Neurons expressing either GluN2B subunits or chimeric GluN2A/GluN2B C-terminus subunits exhibited NMDA-induced superoxide production, whereas neurons expressing chimeric GluN2B/GluN2A C-terminus subunits did not. Neuronal NOX2 activation requires phosphoinositide 3-kinase (PI3K), and NMDA binding to NMDAR increased PI3K association with NMDA GluN2B subunits independent of Ca 2+ influx. These findings identify a non-ionotropic signaling pathway that links NMDAR to NOX2 activation through the C-terminus domain of GluN2B.",
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