Abstract
Sprague-Dawley rats were subjected to a moderate level (2.2 atm) of traumatic brain injury (TBI) using fluid percussion. Injured animals were allowed post-trauma survival periods of 5 min, 3 and 24 h. Regional glutamate receptor subtype binding was assessed with quantitative autoradiography in each group for N-methyl-d-aspartate (NMDA), quisqualate and kainate receptor subpopulations at approximately the -3.8 bregma level and compared to a sham control group. [3H]glutamate binding to the NMDA receptor was significantly (P < 0.05) decreased at 3 h post-TBI in the hippocampal CA1 stratum radiatum, the molecular layers of the dentate gyri and the outer (layers 1-3) and inner (layers 5 and 6) overlying neocortex. NMDA receptor binding was significantly reduced in layers 5 and 6 of the neocortex at all post-trauma survival times but no further differences were seen in the hippocampi. No significant changes were observed with [3H]AMPA binding to quisqualate receptors and [3H]KA binding was significantly reduced only in layers 5 and 6 of the neocortex at 24 h after TBI. These data further confirm the pathological involvemnt of the NMDA receptor complex in brain regions selectively vulnerable to moderate levels of TBI in this model.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 103-107 |
| Number of pages | 5 |
| Journal | Brain Research |
| Volume | 526 |
| Issue number | 1 |
| DOIs | |
| State | Published - Aug 27 1990 |
| Externally published | Yes |
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Keywords
- Autoradiography
- Excitatory amino acid
- Glutamate
- Hippocampus
- Kainate
- N-Methyl-d-aspartate
- Neocortex
- Quisqualate
- Receptor binding
- Selective vulnerability
- Trauma
ASJC Scopus subject areas
- Developmental Biology
- Molecular Biology
- Clinical Neurology
- Neuroscience(all)
Cite this
Excitatory amino acid receptor subtype binding following traumatic brain injury. / Miller, L. P.; Lyeth, Bruce G; Jenkins, L. W.; Oleniak, L.; Panchision, D.; Hamm, R. J.; Phillips, L. L.; Dixon, C. E.; Clifton, G. L.; Hayes, R. L.
In: Brain Research, Vol. 526, No. 1, 27.08.1990, p. 103-107.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Excitatory amino acid receptor subtype binding following traumatic brain injury
AU - Miller, L. P.
AU - Lyeth, Bruce G
AU - Jenkins, L. W.
AU - Oleniak, L.
AU - Panchision, D.
AU - Hamm, R. J.
AU - Phillips, L. L.
AU - Dixon, C. E.
AU - Clifton, G. L.
AU - Hayes, R. L.
PY - 1990/8/27
Y1 - 1990/8/27
N2 - Sprague-Dawley rats were subjected to a moderate level (2.2 atm) of traumatic brain injury (TBI) using fluid percussion. Injured animals were allowed post-trauma survival periods of 5 min, 3 and 24 h. Regional glutamate receptor subtype binding was assessed with quantitative autoradiography in each group for N-methyl-d-aspartate (NMDA), quisqualate and kainate receptor subpopulations at approximately the -3.8 bregma level and compared to a sham control group. [3H]glutamate binding to the NMDA receptor was significantly (P < 0.05) decreased at 3 h post-TBI in the hippocampal CA1 stratum radiatum, the molecular layers of the dentate gyri and the outer (layers 1-3) and inner (layers 5 and 6) overlying neocortex. NMDA receptor binding was significantly reduced in layers 5 and 6 of the neocortex at all post-trauma survival times but no further differences were seen in the hippocampi. No significant changes were observed with [3H]AMPA binding to quisqualate receptors and [3H]KA binding was significantly reduced only in layers 5 and 6 of the neocortex at 24 h after TBI. These data further confirm the pathological involvemnt of the NMDA receptor complex in brain regions selectively vulnerable to moderate levels of TBI in this model.
AB - Sprague-Dawley rats were subjected to a moderate level (2.2 atm) of traumatic brain injury (TBI) using fluid percussion. Injured animals were allowed post-trauma survival periods of 5 min, 3 and 24 h. Regional glutamate receptor subtype binding was assessed with quantitative autoradiography in each group for N-methyl-d-aspartate (NMDA), quisqualate and kainate receptor subpopulations at approximately the -3.8 bregma level and compared to a sham control group. [3H]glutamate binding to the NMDA receptor was significantly (P < 0.05) decreased at 3 h post-TBI in the hippocampal CA1 stratum radiatum, the molecular layers of the dentate gyri and the outer (layers 1-3) and inner (layers 5 and 6) overlying neocortex. NMDA receptor binding was significantly reduced in layers 5 and 6 of the neocortex at all post-trauma survival times but no further differences were seen in the hippocampi. No significant changes were observed with [3H]AMPA binding to quisqualate receptors and [3H]KA binding was significantly reduced only in layers 5 and 6 of the neocortex at 24 h after TBI. These data further confirm the pathological involvemnt of the NMDA receptor complex in brain regions selectively vulnerable to moderate levels of TBI in this model.
KW - Autoradiography
KW - Excitatory amino acid
KW - Glutamate
KW - Hippocampus
KW - Kainate
KW - N-Methyl-d-aspartate
KW - Neocortex
KW - Quisqualate
KW - Receptor binding
KW - Selective vulnerability
KW - Trauma
UR - http://www.scopus.com/inward/record.url?scp=0025121518&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0025121518&partnerID=8YFLogxK
U2 - 10.1016/0006-8993(90)90254-9
DO - 10.1016/0006-8993(90)90254-9
M3 - Article
C2 - 1964103
AN - SCOPUS:0025121518
VL - 526
SP - 103
EP - 107
JO - Brain Research
JF - Brain Research
SN - 0006-8993
IS - 1
ER -