Excitatory amino acid receptor subtype binding following traumatic brain injury

L. P. Miller, Bruce G Lyeth, L. W. Jenkins, L. Oleniak, D. Panchision, R. J. Hamm, L. L. Phillips, C. E. Dixon, G. L. Clifton, R. L. Hayes

Research output: Contribution to journalArticle

107 Citations (Scopus)

Abstract

Sprague-Dawley rats were subjected to a moderate level (2.2 atm) of traumatic brain injury (TBI) using fluid percussion. Injured animals were allowed post-trauma survival periods of 5 min, 3 and 24 h. Regional glutamate receptor subtype binding was assessed with quantitative autoradiography in each group for N-methyl-d-aspartate (NMDA), quisqualate and kainate receptor subpopulations at approximately the -3.8 bregma level and compared to a sham control group. [3H]glutamate binding to the NMDA receptor was significantly (P < 0.05) decreased at 3 h post-TBI in the hippocampal CA1 stratum radiatum, the molecular layers of the dentate gyri and the outer (layers 1-3) and inner (layers 5 and 6) overlying neocortex. NMDA receptor binding was significantly reduced in layers 5 and 6 of the neocortex at all post-trauma survival times but no further differences were seen in the hippocampi. No significant changes were observed with [3H]AMPA binding to quisqualate receptors and [3H]KA binding was significantly reduced only in layers 5 and 6 of the neocortex at 24 h after TBI. These data further confirm the pathological involvemnt of the NMDA receptor complex in brain regions selectively vulnerable to moderate levels of TBI in this model.

Original languageEnglish (US)
Pages (from-to)103-107
Number of pages5
JournalBrain Research
Volume526
Issue number1
DOIs
StatePublished - Aug 27 1990
Externally publishedYes

Fingerprint

Glutamate Receptors
Neocortex
AMPA Receptors
Percussion
Kainic Acid Receptors
Hippocampal CA1 Region
alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid
Dentate Gyrus
Wounds and Injuries
Autoradiography
Sprague Dawley Rats
Glutamic Acid
Hippocampus
Control Groups
Traumatic Brain Injury
aspartic acid receptor
Brain

Keywords

  • Autoradiography
  • Excitatory amino acid
  • Glutamate
  • Hippocampus
  • Kainate
  • N-Methyl-d-aspartate
  • Neocortex
  • Quisqualate
  • Receptor binding
  • Selective vulnerability
  • Trauma

ASJC Scopus subject areas

  • Developmental Biology
  • Molecular Biology
  • Clinical Neurology
  • Neuroscience(all)

Cite this

Miller, L. P., Lyeth, B. G., Jenkins, L. W., Oleniak, L., Panchision, D., Hamm, R. J., ... Hayes, R. L. (1990). Excitatory amino acid receptor subtype binding following traumatic brain injury. Brain Research, 526(1), 103-107. https://doi.org/10.1016/0006-8993(90)90254-9

Excitatory amino acid receptor subtype binding following traumatic brain injury. / Miller, L. P.; Lyeth, Bruce G; Jenkins, L. W.; Oleniak, L.; Panchision, D.; Hamm, R. J.; Phillips, L. L.; Dixon, C. E.; Clifton, G. L.; Hayes, R. L.

In: Brain Research, Vol. 526, No. 1, 27.08.1990, p. 103-107.

Research output: Contribution to journalArticle

Miller, LP, Lyeth, BG, Jenkins, LW, Oleniak, L, Panchision, D, Hamm, RJ, Phillips, LL, Dixon, CE, Clifton, GL & Hayes, RL 1990, 'Excitatory amino acid receptor subtype binding following traumatic brain injury', Brain Research, vol. 526, no. 1, pp. 103-107. https://doi.org/10.1016/0006-8993(90)90254-9
Miller, L. P. ; Lyeth, Bruce G ; Jenkins, L. W. ; Oleniak, L. ; Panchision, D. ; Hamm, R. J. ; Phillips, L. L. ; Dixon, C. E. ; Clifton, G. L. ; Hayes, R. L. / Excitatory amino acid receptor subtype binding following traumatic brain injury. In: Brain Research. 1990 ; Vol. 526, No. 1. pp. 103-107.
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