Abstract
Sprague-Dawley rats were subjected to a moderate level (2.2 atm) of traumatic brain injury (TBI) using fluid percussion. Injured animals were allowed post-trauma survival periods of 5 min, 3 and 24 h. Regional glutamate receptor subtype binding was assessed with quantitative autoradiography in each group for N-methyl-d-aspartate (NMDA), quisqualate and kainate receptor subpopulations at approximately the -3.8 bregma level and compared to a sham control group. [3H]glutamate binding to the NMDA receptor was significantly (P < 0.05) decreased at 3 h post-TBI in the hippocampal CA1 stratum radiatum, the molecular layers of the dentate gyri and the outer (layers 1-3) and inner (layers 5 and 6) overlying neocortex. NMDA receptor binding was significantly reduced in layers 5 and 6 of the neocortex at all post-trauma survival times but no further differences were seen in the hippocampi. No significant changes were observed with [3H]AMPA binding to quisqualate receptors and [3H]KA binding was significantly reduced only in layers 5 and 6 of the neocortex at 24 h after TBI. These data further confirm the pathological involvemnt of the NMDA receptor complex in brain regions selectively vulnerable to moderate levels of TBI in this model.
Original language | English (US) |
---|---|
Pages (from-to) | 103-107 |
Number of pages | 5 |
Journal | Brain Research |
Volume | 526 |
Issue number | 1 |
DOIs | |
State | Published - Aug 27 1990 |
Externally published | Yes |
Keywords
- Autoradiography
- Excitatory amino acid
- Glutamate
- Hippocampus
- Kainate
- N-Methyl-d-aspartate
- Neocortex
- Quisqualate
- Receptor binding
- Selective vulnerability
- Trauma
ASJC Scopus subject areas
- Developmental Biology
- Molecular Biology
- Clinical Neurology
- Neuroscience(all)