Examination of oral cancer biomarkers by tissue microarray analysis

Peter Choi, C. Diana Jordan, Eduardo Mendez, John Houck, Bevan Yueh, D Gregory Farwell, Neal Futran, Chu Chen

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

Objective: To validate the DNA microarray results on a subset of genes that could potentially serve as biomarkers of oral squamous cell carcinoma (OSCC) by examining their expression with an alternate quantitative method and by assessing their protein levels. Design: Based on DNA microarray data from our laboratory and data reported in the literature, we identified 6 potential biomarkers of OSCC to investigate further. We used quantitative real-time polymerase chain reaction to examine expression changes of CDH11, MMP3, SPARC, POSTN, TNC, and TGM3 in OSCC and histologically normal control tissues. We further examined validated markers at the protein level by immunohistochemical analysis of OSCC tissue microarray sections. Results: Quantitative real-time polymerase chain reaction analysis revealed upregulation of CDH11, SPARC, POSTN, and TNC gene expression and decreased TGM3 expression in OSCC tissue compared with control tissue; MMP3 was not found to be differentially expressed. In tissue microarray immunohistochemical analyses, SPARC (secreted protein, acidic, rich in cysteine), periostin, and tenascin C exhibited increased protein expression in tumor tissue compared with control tissue, and their expression was primarily localized within tumor-associated stroma rather than tumor epithelium. Conversely, transglutaminase 3 protein expression was found only within keratinocytes in control tissue and was significantly downregulated in cancer cells. Conclusions: Of 6 potential gene markers of OSCC, initially identified by DNA microarray analyses, differential expression of CDH11, SPARC, POSTN, TNC, and TGM3 were validated by quantitative real-time polymerase chain reaction. Differential expression and localization of proteins encoded by SPARC, POSTN, TNC, and TGM3 were clearly shown by tissue microarray immunohistochemical analysis.

Original languageEnglish (US)
Pages (from-to)539-546
Number of pages8
JournalArchives of Otolaryngology - Head and Neck Surgery
Volume134
Issue number5
DOIs
StatePublished - May 2008

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Tissue Array Analysis
Mouth Neoplasms
Tumor Biomarkers
Squamous Cell Carcinoma
Cysteine
Proteins
Oligonucleotide Array Sequence Analysis
Real-Time Polymerase Chain Reaction
Neoplasms
Biomarkers
Tenascin
Transglutaminases
Microarray Analysis
Keratinocytes
Genes
Up-Regulation
Down-Regulation
Epithelium

ASJC Scopus subject areas

  • Otorhinolaryngology

Cite this

Examination of oral cancer biomarkers by tissue microarray analysis. / Choi, Peter; Jordan, C. Diana; Mendez, Eduardo; Houck, John; Yueh, Bevan; Farwell, D Gregory; Futran, Neal; Chen, Chu.

In: Archives of Otolaryngology - Head and Neck Surgery, Vol. 134, No. 5, 05.2008, p. 539-546.

Research output: Contribution to journalArticle

Choi, Peter ; Jordan, C. Diana ; Mendez, Eduardo ; Houck, John ; Yueh, Bevan ; Farwell, D Gregory ; Futran, Neal ; Chen, Chu. / Examination of oral cancer biomarkers by tissue microarray analysis. In: Archives of Otolaryngology - Head and Neck Surgery. 2008 ; Vol. 134, No. 5. pp. 539-546.
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