Examination of galectins in phagocytosis

Huan Yuan Chen, I. Chun Weng, Chi Shan Li, Lei Wan, Fu Tong Liu

Research output: Contribution to journalArticle

8 Scopus citations

Abstract

Galectins, a family of β-galactoside-binding proteins, are expressed in many different phagocytic leukocytes (granulocytes, monocytes, and macrophages). A number of family members have been shown to play an important role in ingestion of particles (phagocytosis), thus contributing to clearance of damaged cells and host defense against pathogens. Here we describe procedures for analysis of the roles of galectins in phagocytosis by using galectin-3 as an example. We emphasize the function of endogenous galectin-3 as determined by comparison of phagocytosis by macrophages from galectin-3 knockout mice and wild-type mice. We focus on the role of galectin-3 in phagocytosis of pathogens and Fcγ receptor-mediated phagocytosis of opsonized cells and particles.

Original languageEnglish (US)
Pages (from-to)201-213
Number of pages13
JournalMethods in Molecular Biology
Volume1207
DOIs
StatePublished - 2015

Keywords

  • Galectin
  • Macrophage
  • Opsonization
  • Phagocytosis

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Medicine(all)

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    Chen, H. Y., Weng, I. C., Li, C. S., Wan, L., & Liu, F. T. (2015). Examination of galectins in phagocytosis. Methods in Molecular Biology, 1207, 201-213. https://doi.org/10.1007/978-1-4939-1396-1_13