Exact mass determination for elemental analysis of ions produced by matrix-assisted laser desorption

Jiangyue Wu, Steven T. Fannin, Melanie A. Franklin, Tadeusz F. Molinski, Carlito B Lebrilla

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

The exact masses of bastadins, cyclic peptides from marine sponges Ianthella basta, are determined using matrix-assisted laser desorption ionization (MALDI) coupled to a Fourier transform mass spectrometer. Two known compounds were mixed with the unknown to serve as internal calibrants. The mass of the calibrants bracketed the mass of the unknown compound. With this method, exact masses were obtained to within 5 ppm for single determinations, and less than 3 ppm for multiple determinations, allowing the derivation of elemental composition. This method is viable for routinely obtaining the exact masses of new compounds with MALDI.

Original languageEnglish (US)
Pages (from-to)3788-3792
Number of pages5
JournalAnalytical Chemistry
Volume67
Issue number20
StatePublished - Oct 15 1995

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Ionization
Desorption
Ions
Cyclic Peptides
Lasers
Mass spectrometers
Chemical analysis
Fourier transforms
phosphinothricin

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Wu, J., Fannin, S. T., Franklin, M. A., Molinski, T. F., & Lebrilla, C. B. (1995). Exact mass determination for elemental analysis of ions produced by matrix-assisted laser desorption. Analytical Chemistry, 67(20), 3788-3792.

Exact mass determination for elemental analysis of ions produced by matrix-assisted laser desorption. / Wu, Jiangyue; Fannin, Steven T.; Franklin, Melanie A.; Molinski, Tadeusz F.; Lebrilla, Carlito B.

In: Analytical Chemistry, Vol. 67, No. 20, 15.10.1995, p. 3788-3792.

Research output: Contribution to journalArticle

Wu, J, Fannin, ST, Franklin, MA, Molinski, TF & Lebrilla, CB 1995, 'Exact mass determination for elemental analysis of ions produced by matrix-assisted laser desorption', Analytical Chemistry, vol. 67, no. 20, pp. 3788-3792.
Wu J, Fannin ST, Franklin MA, Molinski TF, Lebrilla CB. Exact mass determination for elemental analysis of ions produced by matrix-assisted laser desorption. Analytical Chemistry. 1995 Oct 15;67(20):3788-3792.
Wu, Jiangyue ; Fannin, Steven T. ; Franklin, Melanie A. ; Molinski, Tadeusz F. ; Lebrilla, Carlito B. / Exact mass determination for elemental analysis of ions produced by matrix-assisted laser desorption. In: Analytical Chemistry. 1995 ; Vol. 67, No. 20. pp. 3788-3792.
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