Exact mass determination for elemental analysis of ions produced by matrix-assisted laser desorption

Jiangyue Wu, Steven T. Fannin, Melanie A. Franklin, Tadeusz F. Molinski, Carlito B Lebrilla

Research output: Contribution to journalArticle

22 Scopus citations

Abstract

The exact masses of bastadins, cyclic peptides from marine sponges Ianthella basta, are determined using matrix-assisted laser desorption ionization (MALDI) coupled to a Fourier transform mass spectrometer. Two known compounds were mixed with the unknown to serve as internal calibrants. The mass of the calibrants bracketed the mass of the unknown compound. With this method, exact masses were obtained to within 5 ppm for single determinations, and less than 3 ppm for multiple determinations, allowing the derivation of elemental composition. This method is viable for routinely obtaining the exact masses of new compounds with MALDI.

Original languageEnglish (US)
Pages (from-to)3788-3792
Number of pages5
JournalAnalytical Chemistry
Volume67
Issue number20
StatePublished - Oct 15 1995

ASJC Scopus subject areas

  • Analytical Chemistry

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