Ex Vivo Analysis of Tryptophan Metabolism Using 19F NMR

Robert J. Tombari, Carla M. Saunders, Chun Yi Wu, Lee E. Dunlap, Dean J. Tantillo, David Olson

Research output: Contribution to journalArticle

Abstract

Tryptophan, an essential amino acid, is metabolized into a variety of small molecules capable of impacting human physiology, and aberrant tryptophan metabolism has been linked to a number of diseases. There are three principal routes by which tryptophan is degraded, and thus methods for measuring metabolic flux through these pathways can be used to understand the factors that perturb tryptophan metabolism and potentially to measure disease biomarkers. Here, we describe a method utilizing 6-fluorotryptophan as a probe for detecting tryptophan metabolites in ex vivo tissue samples via 19F nuclear magnetic resonance. As a proof of concept, we demonstrate that 6-fluorotryptophan can be used to measure changes in tryptophan metabolism resulting from antibiotic-induced changes in gut microbiota composition. Taken together, we describe a general strategy for monitoring amino acid metabolism using 19F nuclear magnetic resonance that is operationally simple and does not require chromatographic separation of metabolites.

Original languageEnglish (US)
JournalACS Chemical Biology
DOIs
StateAccepted/In press - Jan 1 2019

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Metabolism
Tryptophan
Nuclear magnetic resonance
Metabolites
Magnetic Resonance Spectroscopy
Essential Amino Acids
Physiology
Biomarkers
Tissue
Fluxes
Anti-Bacterial Agents
Amino Acids
Molecules
Monitoring
Chemical analysis

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine

Cite this

Tombari, R. J., Saunders, C. M., Wu, C. Y., Dunlap, L. E., Tantillo, D. J., & Olson, D. (Accepted/In press). Ex Vivo Analysis of Tryptophan Metabolism Using 19F NMR. ACS Chemical Biology. https://doi.org/10.1021/acschembio.9b00548

Ex Vivo Analysis of Tryptophan Metabolism Using 19F NMR. / Tombari, Robert J.; Saunders, Carla M.; Wu, Chun Yi; Dunlap, Lee E.; Tantillo, Dean J.; Olson, David.

In: ACS Chemical Biology, 01.01.2019.

Research output: Contribution to journalArticle

Tombari, Robert J. ; Saunders, Carla M. ; Wu, Chun Yi ; Dunlap, Lee E. ; Tantillo, Dean J. ; Olson, David. / Ex Vivo Analysis of Tryptophan Metabolism Using 19F NMR. In: ACS Chemical Biology. 2019.
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