Evolutionary relationship between Old World West Nile virus strains: Evidence for viral gene flow between Africa, the Middle East, and Europe

R. N. Charrel, Aaron Brault, P. Gallian, J. J. Lemasson, B. Murgue, S. Murri, B. Pastorino, H. Zeller, R. De Chesse, P. De Micco, X. De Lamballerie

Research output: Contribution to journalArticle

100 Citations (Scopus)

Abstract

Little is known about the genetic relationships between European and other Old-World strains of West Nile virus (WNV) and persistence of WNV North of Mediterranean. We characterized the complete genomes of three WNV strains from France (horse-2000), Tunisia (human-1997) and Kenya (mosquito-1998), and the envelope, NS3 and NS5 genes of the Koutango virus. Phylogenetic analyses including all available full-length sequences showed that: (1) Koutango virus is a distant variant of WNV; (2) the three characterized strains belong to lineage 1, clade 1a; (3) the Tunisian strain roots the lineage of viruses introduced in North America. We established that currently available partial envelope sequences do not generate reliable phylogenies. Accordingly, establishing a large WNV sequence database is pivotal for the understanding of spatial and temporal epidemiology of this virus. For rapid completion of that purpose, colinearized E-NS3-NS5 gene sequences were shown to constitute a valuable surrogate for complete sequences.

Original languageEnglish (US)
Pages (from-to)381-388
Number of pages8
JournalVirology
Volume315
Issue number2
DOIs
StatePublished - Oct 25 2003
Externally publishedYes

Fingerprint

West Nile virus
Middle East
Gene Flow
Viral Genes
Viruses
Tunisia
Kenya
Phylogeny
North America
Culicidae
Genes
Horses
France
Epidemiology
Genome
Databases

ASJC Scopus subject areas

  • Virology

Cite this

Evolutionary relationship between Old World West Nile virus strains : Evidence for viral gene flow between Africa, the Middle East, and Europe. / Charrel, R. N.; Brault, Aaron; Gallian, P.; Lemasson, J. J.; Murgue, B.; Murri, S.; Pastorino, B.; Zeller, H.; De Chesse, R.; De Micco, P.; De Lamballerie, X.

In: Virology, Vol. 315, No. 2, 25.10.2003, p. 381-388.

Research output: Contribution to journalArticle

Charrel, RN, Brault, A, Gallian, P, Lemasson, JJ, Murgue, B, Murri, S, Pastorino, B, Zeller, H, De Chesse, R, De Micco, P & De Lamballerie, X 2003, 'Evolutionary relationship between Old World West Nile virus strains: Evidence for viral gene flow between Africa, the Middle East, and Europe', Virology, vol. 315, no. 2, pp. 381-388. https://doi.org/10.1016/S0042-6822(03)00536-1
Charrel, R. N. ; Brault, Aaron ; Gallian, P. ; Lemasson, J. J. ; Murgue, B. ; Murri, S. ; Pastorino, B. ; Zeller, H. ; De Chesse, R. ; De Micco, P. ; De Lamballerie, X. / Evolutionary relationship between Old World West Nile virus strains : Evidence for viral gene flow between Africa, the Middle East, and Europe. In: Virology. 2003 ; Vol. 315, No. 2. pp. 381-388.
@article{fec44cb2e4f048bbbd508256132002aa,
title = "Evolutionary relationship between Old World West Nile virus strains: Evidence for viral gene flow between Africa, the Middle East, and Europe",
abstract = "Little is known about the genetic relationships between European and other Old-World strains of West Nile virus (WNV) and persistence of WNV North of Mediterranean. We characterized the complete genomes of three WNV strains from France (horse-2000), Tunisia (human-1997) and Kenya (mosquito-1998), and the envelope, NS3 and NS5 genes of the Koutango virus. Phylogenetic analyses including all available full-length sequences showed that: (1) Koutango virus is a distant variant of WNV; (2) the three characterized strains belong to lineage 1, clade 1a; (3) the Tunisian strain roots the lineage of viruses introduced in North America. We established that currently available partial envelope sequences do not generate reliable phylogenies. Accordingly, establishing a large WNV sequence database is pivotal for the understanding of spatial and temporal epidemiology of this virus. For rapid completion of that purpose, colinearized E-NS3-NS5 gene sequences were shown to constitute a valuable surrogate for complete sequences.",
author = "Charrel, {R. N.} and Aaron Brault and P. Gallian and Lemasson, {J. J.} and B. Murgue and S. Murri and B. Pastorino and H. Zeller and {De Chesse}, R. and {De Micco}, P. and {De Lamballerie}, X.",
year = "2003",
month = "10",
day = "25",
doi = "10.1016/S0042-6822(03)00536-1",
language = "English (US)",
volume = "315",
pages = "381--388",
journal = "Virology",
issn = "0042-6822",
publisher = "Academic Press Inc.",
number = "2",

}

TY - JOUR

T1 - Evolutionary relationship between Old World West Nile virus strains

T2 - Evidence for viral gene flow between Africa, the Middle East, and Europe

AU - Charrel, R. N.

AU - Brault, Aaron

AU - Gallian, P.

AU - Lemasson, J. J.

AU - Murgue, B.

AU - Murri, S.

AU - Pastorino, B.

AU - Zeller, H.

AU - De Chesse, R.

AU - De Micco, P.

AU - De Lamballerie, X.

PY - 2003/10/25

Y1 - 2003/10/25

N2 - Little is known about the genetic relationships between European and other Old-World strains of West Nile virus (WNV) and persistence of WNV North of Mediterranean. We characterized the complete genomes of three WNV strains from France (horse-2000), Tunisia (human-1997) and Kenya (mosquito-1998), and the envelope, NS3 and NS5 genes of the Koutango virus. Phylogenetic analyses including all available full-length sequences showed that: (1) Koutango virus is a distant variant of WNV; (2) the three characterized strains belong to lineage 1, clade 1a; (3) the Tunisian strain roots the lineage of viruses introduced in North America. We established that currently available partial envelope sequences do not generate reliable phylogenies. Accordingly, establishing a large WNV sequence database is pivotal for the understanding of spatial and temporal epidemiology of this virus. For rapid completion of that purpose, colinearized E-NS3-NS5 gene sequences were shown to constitute a valuable surrogate for complete sequences.

AB - Little is known about the genetic relationships between European and other Old-World strains of West Nile virus (WNV) and persistence of WNV North of Mediterranean. We characterized the complete genomes of three WNV strains from France (horse-2000), Tunisia (human-1997) and Kenya (mosquito-1998), and the envelope, NS3 and NS5 genes of the Koutango virus. Phylogenetic analyses including all available full-length sequences showed that: (1) Koutango virus is a distant variant of WNV; (2) the three characterized strains belong to lineage 1, clade 1a; (3) the Tunisian strain roots the lineage of viruses introduced in North America. We established that currently available partial envelope sequences do not generate reliable phylogenies. Accordingly, establishing a large WNV sequence database is pivotal for the understanding of spatial and temporal epidemiology of this virus. For rapid completion of that purpose, colinearized E-NS3-NS5 gene sequences were shown to constitute a valuable surrogate for complete sequences.

UR - http://www.scopus.com/inward/record.url?scp=0142185268&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0142185268&partnerID=8YFLogxK

U2 - 10.1016/S0042-6822(03)00536-1

DO - 10.1016/S0042-6822(03)00536-1

M3 - Article

C2 - 14585341

AN - SCOPUS:0142185268

VL - 315

SP - 381

EP - 388

JO - Virology

JF - Virology

SN - 0042-6822

IS - 2

ER -