Evolution of nef variants in gut associated lymphoid tissue of rhesus macaques during primary simian immunodeficiency virus infection

Thomas Ndolo, Michael Syvanen, Thomas Ellison, Satya Dandekar

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

We utilized the simian immunodeficiency virus model of AIDS to examine evolution of nef gene in gut-associated lymphoid tissue (GALT) during primary and early asymptomatic stages of infection. Macaques were infected with a cloned virus, SIVmac239/nef-stop harboring a premature stop codon in the nef gene. Restoration of the nef open reading frame occurred in GALT early at 3 days post-infection. Analysis of nef sequences by phylogenetic tools showed that evolution of nef was neutral thereafter, as evidenced by the ratio of synonymous to nonsynonymous substitutions, a star pattern in unrooted trees and distribution of amino acid replacements fitting a simple Poisson process. Two regions encoding for a nuclear localization signal and a CTL epitope were conserved. Thus, GALT was a site for strong positive selection of functional nef during initial stages of infection. However, evolution of the nef gene thereafter was neutral during early asymptomatic stage of infection.

Original languageEnglish (US)
Pages (from-to)1-11
Number of pages11
JournalVirology
Volume343
Issue number1
DOIs
StatePublished - Dec 5 2005

Keywords

  • Adaptive
  • Evolution
  • GALT
  • Neutral model
  • Positive selection
  • SIV-Nef
  • Synonymous and nonsynonymous

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Fingerprint Dive into the research topics of 'Evolution of nef variants in gut associated lymphoid tissue of rhesus macaques during primary simian immunodeficiency virus infection'. Together they form a unique fingerprint.

Cite this