Evolution of metastasis revealed by mutational landscapes of chemically induced skin cancers

Melissa Q. McCreery, Kyle D. Halliwill, Douglas Chin, Reyno Delrosario, Gillian Hirst, Peter Vuong, Kuang-Yu Jen, James Hewinson, David J. Adams, Allan Balmain

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

Human tumors show a high level of genetic heterogeneity, but the processes that influence the timing and route of metastatic dissemination of the subclones are unknown. Here we have used whole-exome sequencing of 103 matched benign, malignant and metastatic skin tumors from genetically heterogeneous mice to demonstrate that most metastases disseminate synchronously from the primary tumor, supporting parallel rather than linear evolution as the predominant model of metastasis. Shared mutations between primary carcinomas and their matched metastases have the distinct A-to-T signature of the initiating carcinogen dimethylbenzanthracene, but non-shared mutations are primarily G-to-T, a signature associated with oxidative stress. The existence of carcinomas that either did or did not metastasize in the same host animal suggests that there are tumor-intrinsic factors that influence metastatic seeding. We also demonstrate the importance of germline polymorphisms in determining allele-specific mutations, and we identify somatic genetic alterations that are specifically related to initiation of carcinogenesis by Hras or Kras mutations. Mouse tumors that mimic the genetic heterogeneity of human cancers can aid our understanding of the clonal evolution of metastasis and provide a realistic model for the testing of novel therapies.

Original languageEnglish (US)
Pages (from-to)1514-1520
Number of pages7
JournalNature Medicine
Volume21
Issue number12
DOIs
StatePublished - Dec 1 2015
Externally publishedYes

Fingerprint

Skin Neoplasms
Tumors
Skin
Neoplasm Metastasis
Neoplasms
Mutation
Genetic Heterogeneity
Intrinsic Factor
Genetic Phenomena
Oxidative stress
Clonal Evolution
Exome
Carcinoma
Polymorphism
Carcinogens
Animals
Carcinogenesis
Oxidative Stress
Alleles
Testing

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

McCreery, M. Q., Halliwill, K. D., Chin, D., Delrosario, R., Hirst, G., Vuong, P., ... Balmain, A. (2015). Evolution of metastasis revealed by mutational landscapes of chemically induced skin cancers. Nature Medicine, 21(12), 1514-1520. https://doi.org/10.1038/nm.3979

Evolution of metastasis revealed by mutational landscapes of chemically induced skin cancers. / McCreery, Melissa Q.; Halliwill, Kyle D.; Chin, Douglas; Delrosario, Reyno; Hirst, Gillian; Vuong, Peter; Jen, Kuang-Yu; Hewinson, James; Adams, David J.; Balmain, Allan.

In: Nature Medicine, Vol. 21, No. 12, 01.12.2015, p. 1514-1520.

Research output: Contribution to journalArticle

McCreery, MQ, Halliwill, KD, Chin, D, Delrosario, R, Hirst, G, Vuong, P, Jen, K-Y, Hewinson, J, Adams, DJ & Balmain, A 2015, 'Evolution of metastasis revealed by mutational landscapes of chemically induced skin cancers', Nature Medicine, vol. 21, no. 12, pp. 1514-1520. https://doi.org/10.1038/nm.3979
McCreery MQ, Halliwill KD, Chin D, Delrosario R, Hirst G, Vuong P et al. Evolution of metastasis revealed by mutational landscapes of chemically induced skin cancers. Nature Medicine. 2015 Dec 1;21(12):1514-1520. https://doi.org/10.1038/nm.3979
McCreery, Melissa Q. ; Halliwill, Kyle D. ; Chin, Douglas ; Delrosario, Reyno ; Hirst, Gillian ; Vuong, Peter ; Jen, Kuang-Yu ; Hewinson, James ; Adams, David J. ; Balmain, Allan. / Evolution of metastasis revealed by mutational landscapes of chemically induced skin cancers. In: Nature Medicine. 2015 ; Vol. 21, No. 12. pp. 1514-1520.
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