Evolution of host adaptation in the Salmonella typhoid toxin

Xiang Gao, Lingquan Deng, Gabrielle Stack, Hai Yu, Xi Chen, Yuko Naito-Matsui, Ajit Varki, Jorge E. Galán

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The evolution of virulence traits is central for the emergence or re-emergence of microbial pathogens and for their adaptation to a specific host1–5. Typhoid toxin is an essential virulence factor of the human-adapted bacterial pathogen Salmonella Typhi6,7, the cause of typhoid fever in humans8–12. Typhoid toxin has a unique A2B5 architecture with two covalently linked enzymatic ‘A’ subunits, PltA and CdtB, associated with a homopentameric ‘B’ subunit made up of PltB, which has binding specificity for the N-acetylneuraminic acid (Neu5Ac) sialoglycans6,13 predominantly present in humans14. Here, we examine the functional and structural relationship between typhoid toxin and ArtAB, an evolutionarily related AB5 toxin encoded by the broad-host Salmonella Typhimurium15. We find that ArtA and ArtB, homologues of PltA and PltB, can form a functional complex with the typhoid toxin CdtB subunit after substitution of a single amino acid in ArtA, while ArtB can form a functional complex with wild-type PltA and CdtB. We also found that, after addition of a single-terminal Cys residue, a CdtB homologue from cytolethal distending toxin can form a functional complex with ArtA and ArtB. In line with the broad host specificity of S. Typhimurium, we found that ArtB binds human glycans, terminated in N-acetylneuraminic acid, as well as glycans terminated in N-glycolylneuraminic acid (Neu5Gc), which are expressed in most other mammals14. The atomic structure of ArtB bound to its receptor shows the presence of an additional glycan-binding site, which broadens its binding specificity. Despite equivalent toxicity in vitro, we found that the ArtB/PltA/CdtB chimaeric toxin exhibits reduced lethality in an animal model, indicating that the host specialization of typhoid toxin has optimized its targeting mechanisms to the human host. This is a remarkable example of a toxin evolving to broaden its enzymatic activities and adapt to a specific host.

Original languageEnglish (US)
Pages (from-to)1-8
Number of pages8
JournalNature Microbiology
DOIs
StateAccepted/In press - Oct 9 2017

Fingerprint

Typhoid Fever
Polysaccharides
N-Acetylneuraminic Acid
Salmonella
Immunotoxins
Host Specificity
Virulence Factors
Virulence
salmonella toxin
Animal Models
Binding Sites
Amino Acids

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Applied Microbiology and Biotechnology
  • Genetics
  • Microbiology (medical)
  • Cell Biology

Cite this

Gao, X., Deng, L., Stack, G., Yu, H., Chen, X., Naito-Matsui, Y., ... Galán, J. E. (Accepted/In press). Evolution of host adaptation in the Salmonella typhoid toxin. Nature Microbiology, 1-8. https://doi.org/10.1038/s41564-017-0033-2

Evolution of host adaptation in the Salmonella typhoid toxin. / Gao, Xiang; Deng, Lingquan; Stack, Gabrielle; Yu, Hai; Chen, Xi; Naito-Matsui, Yuko; Varki, Ajit; Galán, Jorge E.

In: Nature Microbiology, 09.10.2017, p. 1-8.

Research output: Contribution to journalArticle

Gao, X, Deng, L, Stack, G, Yu, H, Chen, X, Naito-Matsui, Y, Varki, A & Galán, JE 2017, 'Evolution of host adaptation in the Salmonella typhoid toxin', Nature Microbiology, pp. 1-8. https://doi.org/10.1038/s41564-017-0033-2
Gao, Xiang ; Deng, Lingquan ; Stack, Gabrielle ; Yu, Hai ; Chen, Xi ; Naito-Matsui, Yuko ; Varki, Ajit ; Galán, Jorge E. / Evolution of host adaptation in the Salmonella typhoid toxin. In: Nature Microbiology. 2017 ; pp. 1-8.
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