Evolution of coenzyme B12 synthesis among enteric bacteria: Evidence for loss and reacquisition of a multigene complex

Jeffrey G. Lawrence, John R. Roth

Research output: Contribution to journalArticle

80 Citations (Scopus)

Abstract

We have examined the distribution of cobalamin (coenzyme B12) synthetic ability and cobalamin-dependent metabolism among enteric bacteria. Most species of enteric bacteria tested synthesize cobalamin under both aerobic and anaerobic conditions and ferment glycerol in a cobalamin-dependent fashion. The group of species including Escherichia coli and Salmonella typhimurium cannot ferment glycerol. E. coli strains cannot synthesize cobalamin de novo, and Salmonella spp. synthesize cobalamin only under anaerobic conditions. In addition, the cobalamin synthetic genes of Salmonella spp. (cob) show a regulatory pattern different from that of other enteric taxa tested. We propose that the cobalamin synthetic genes, as well as genes providing cobalamin-dependent diol dehydratase, were lost by a common ancestor of E. coli and Salmonella spp. and were reintroduced as a single fragment into the Salmonella lineage from an exogenous source. Consistent with this hypothesis, the S. typhimurium cob genes do not hybridize with the genomes of other enteric species. The Salmonella cob operon may represent a class of genes characterized by periodic loss and reacquisition by host genomes. This process may be an important aspect of bacterial population genetics and evolution.

Original languageEnglish (US)
Pages (from-to)11-24
Number of pages14
JournalGenetics
Volume142
Issue number1
StatePublished - Jan 1996
Externally publishedYes

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Enterobacteriaceae
Vitamin B 12
Salmonella
Synthetic Genes
Salmonella typhimurium
Escherichia coli
Glycerol
Propanediol Dehydratase
Genome
Genes
cobamamide
Molecular Evolution
Population Genetics
Operon

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)

Cite this

Evolution of coenzyme B12 synthesis among enteric bacteria : Evidence for loss and reacquisition of a multigene complex. / Lawrence, Jeffrey G.; Roth, John R.

In: Genetics, Vol. 142, No. 1, 01.1996, p. 11-24.

Research output: Contribution to journalArticle

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