Evolution of antigen variation in the tick-borne pathogen anaplasma phagocytophilum

Daniel Rejmanek, Patrick Foley, Anthony Barbet, Janet E Foley

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Anaplasma phagocytophilum is an obligately intracellular tick-Transmitted bacterial pathogen of humans and other animals. During the course of infection, A. phagocytophilum utilizes gene conversion to shuffle ;100 functional pseudogenes into a single expression cassette of the msp2(p44) gene, which codes for the major surface antigen and major surface protein 2 (MSP2). The role and extent of msp2(p44) recombination, particularly in hosts that only experience acute infections, is not clear. In the present study, we explored patterns of recombination and expression of the msp2(p44) gene of A. phagocytophilum in a serially infected mouse model. Even though the bacterium was passed rapidly among mice, minimizing the opportunities for the host to develop adaptive immunity, we detected the emergence of 34 unique msp2(p44) expression cassette variants. The expression of msp2(p44) pseudogenes did not follow a consistent pattern among different groups of mice, although some pseudogenes were expressed more frequently than others. In addition, among 263 expressed pseudogenes, 3 mosaic sequences each consisting of 2 different pseudogenes were identified. Population genetic analysis showed that genetic diversity and subpopulation differentiation tended to increase over time until stationarity was reached but that the variance that was observed in allele (expressed pseudogene) frequency could occur by drift alone only if a high variance in bacterial reproduction could be assumed. These findings suggest that evolutionary forces influencing antigen variation in A. phagocytophilum may comprise random genetic drift as well as some innate but apparently nonpurifying selection prior to the strong frequency-dependent selection that occurs cyclically after hosts develop strong adaptive immunity.

Original languageEnglish (US)
Pages (from-to)391-400
Number of pages10
JournalMolecular Biology and Evolution
Volume29
Issue number1
DOIs
StatePublished - Jan 2012

Fingerprint

Anaplasma phagocytophilum
Antigenic Variation
Pseudogenes
pseudogenes
tick
Ticks
antigen
ticks
pathogen
immunity
antigens
recombination
gene
pathogens
genetic drift
genetic analysis
Adaptive Immunity
subpopulation
Genetic Recombination
population genetics

Keywords

  • Anaplasma phagocytophilum
  • Antigen variation
  • Drift
  • Msp2(p44)
  • Recombination
  • Selection

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Ecology, Evolution, Behavior and Systematics

Cite this

Evolution of antigen variation in the tick-borne pathogen anaplasma phagocytophilum. / Rejmanek, Daniel; Foley, Patrick; Barbet, Anthony; Foley, Janet E.

In: Molecular Biology and Evolution, Vol. 29, No. 1, 01.2012, p. 391-400.

Research output: Contribution to journalArticle

Rejmanek, Daniel ; Foley, Patrick ; Barbet, Anthony ; Foley, Janet E. / Evolution of antigen variation in the tick-borne pathogen anaplasma phagocytophilum. In: Molecular Biology and Evolution. 2012 ; Vol. 29, No. 1. pp. 391-400.
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