Evolution and recombination of genes encoding HIV-1 drug resistance and tropism during antiretroviral therapy

Binshan Shi, Christina Kitchen, Barbara Weiser, Douglas Mayers, Brian Foley, Kimdar Kemal, Kathryn Anastos, Marc Suchard, Monica Parker, Cheryl Brunner, Harold Burger

Research output: Contribution to journalArticle

23 Scopus citations

Abstract

Characterization of residual plasma virus during antiretroviral therapy (ART) is a high priority to improve understanding of HIV-1 pathogenesis and therapy. To understand the evolution of HIV-1 pol and env genes in viremic patients under selective pressure of ART, we performed longitudinal analyses of plasma-derived pol and env sequences from single HIV-1 genomes. We tested the hypotheses that drug resistance in pol was unrelated to changes in coreceptor usage (tropism), and that recombination played a role in evolution of viral strains. Recombinants were identified by using Bayesian and other computational methods. High-level genotypic resistance was seen in ~70% of X4 and R5 strains during ART. There was no significant association between resistance and tropism. Each patient displayed at least one recombinant encompassing env and representing a change in predicted tropism. These data suggest that, in addition to mutation, recombination can play a significant role in shaping HIV-1 evolution.

Original languageEnglish (US)
Pages (from-to)5-20
Number of pages16
JournalVirology
Volume404
Issue number1
DOIs
StatePublished - Aug 2010
Externally publishedYes

Keywords

  • HIV-1 drug resistance
  • HIV-1 recombination
  • HIV-1 tropism

ASJC Scopus subject areas

  • Virology

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    Shi, B., Kitchen, C., Weiser, B., Mayers, D., Foley, B., Kemal, K., Anastos, K., Suchard, M., Parker, M., Brunner, C., & Burger, H. (2010). Evolution and recombination of genes encoding HIV-1 drug resistance and tropism during antiretroviral therapy. Virology, 404(1), 5-20. https://doi.org/10.1016/j.virol.2010.04.008