TY - JOUR
T1 - Evidence that the antigen of autologous immune complex glomerulonephritis of rats is a mannose- or glucose-containing glycoprotein
AU - Makker, Sudesh P
PY - 1980
Y1 - 1980
N2 - Chemical nature of the brush border antigen (BBAg) of the autologous immune complex glomerulonephritis (AIC) of rats was investigated by a histochemical method utilizing indirect immunofluorescence. Fresh frozen rat kidney sections, a specific antibody to BBAg (BBAb), purified lectins (concanavalin A, Ricin II, wheat germ hemagglutinin) and a specific sugar, α-methyl-D-mannopyranoside were used. The reaction of the specific antibody BBAb to its antigen BBAg on tissue sections was blocked by concanavalin A but not by Ricin II and wheat germ agglutinin. This blockade was reversed by α-methyl-D-mannopyranoside. These results suggest that the antigen of AIC of rats is a mannose or glucose containing glycoprotein.
AB - Chemical nature of the brush border antigen (BBAg) of the autologous immune complex glomerulonephritis (AIC) of rats was investigated by a histochemical method utilizing indirect immunofluorescence. Fresh frozen rat kidney sections, a specific antibody to BBAg (BBAb), purified lectins (concanavalin A, Ricin II, wheat germ hemagglutinin) and a specific sugar, α-methyl-D-mannopyranoside were used. The reaction of the specific antibody BBAb to its antigen BBAg on tissue sections was blocked by concanavalin A but not by Ricin II and wheat germ agglutinin. This blockade was reversed by α-methyl-D-mannopyranoside. These results suggest that the antigen of AIC of rats is a mannose or glucose containing glycoprotein.
UR - http://www.scopus.com/inward/record.url?scp=0019154493&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0019154493&partnerID=8YFLogxK
M3 - Article
C2 - 6444339
AN - SCOPUS:0019154493
VL - 163
SP - 95
EP - 99
JO - Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N. Y.)
JF - Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N. Y.)
SN - 1535-3702
IS - 1
ER -