Evidence that F plasmid transfer replication underlies apparent adaptive mutation

Timothy Galitski, John R. Roth

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Abstract

An Escherichia coli K12 strain, FC40, has been used extensively in the analysis of adaptive mutability. This strain carries a revertible mutant lac allele on an F plasmid and accumulates Lac+ (lactose utilizing) revertants, but not unselected mutants, when placed on selective medium. These adaptive mutations are a subset of spontaneous types and their formation depends on the RecABC functions. Data presented here suggest that this phenomenon depends on transfer functions of the F factor. Fertility inhibition eliminates RecA-dependent adaptive reversion. Thus, "adaptive" revertants may form during replication from the transfer origin, whereas loci in the nonreplicating chromosome show little mutation.

Original languageEnglish (US)
Pages (from-to)421-423
Number of pages3
JournalScience
Volume268
Issue number5209
StatePublished - 1995
Externally publishedYes

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Cite this

Galitski, T., & Roth, J. R. (1995). Evidence that F plasmid transfer replication underlies apparent adaptive mutation. Science, 268(5209), 421-423.