Evidence that F plasmid transfer replication underlies apparent adaptive mutation

Timothy Galitski, John R. Roth

Research output: Contribution to journalArticle

110 Citations (Scopus)

Abstract

An Escherichia coli K12 strain, FC40, has been used extensively in the analysis of adaptive mutability. This strain carries a revertible mutant lac allele on an F plasmid and accumulates Lac+ (lactose utilizing) revertants, but not unselected mutants, when placed on selective medium. These adaptive mutations are a subset of spontaneous types and their formation depends on the RecABC functions. Data presented here suggest that this phenomenon depends on transfer functions of the F factor. Fertility inhibition eliminates RecA-dependent adaptive reversion. Thus, "adaptive" revertants may form during replication from the transfer origin, whereas loci in the nonreplicating chromosome show little mutation.

Original languageEnglish (US)
Pages (from-to)421-423
Number of pages3
JournalScience
Volume268
Issue number5209
StatePublished - 1995
Externally publishedYes

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F Factor
Escherichia coli K12
Mutation
Replication Origin
Lactose
Fertility
Chromosomes
Alleles

ASJC Scopus subject areas

  • General

Cite this

Galitski, T., & Roth, J. R. (1995). Evidence that F plasmid transfer replication underlies apparent adaptive mutation. Science, 268(5209), 421-423.

Evidence that F plasmid transfer replication underlies apparent adaptive mutation. / Galitski, Timothy; Roth, John R.

In: Science, Vol. 268, No. 5209, 1995, p. 421-423.

Research output: Contribution to journalArticle

Galitski, T & Roth, JR 1995, 'Evidence that F plasmid transfer replication underlies apparent adaptive mutation', Science, vol. 268, no. 5209, pp. 421-423.
Galitski, Timothy ; Roth, John R. / Evidence that F plasmid transfer replication underlies apparent adaptive mutation. In: Science. 1995 ; Vol. 268, No. 5209. pp. 421-423.
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