Abstract
An Escherichia coli K12 strain, FC40, has been used extensively in the analysis of adaptive mutability. This strain carries a revertible mutant lac allele on an F plasmid and accumulates Lac+ (lactose utilizing) revertants, but not unselected mutants, when placed on selective medium. These adaptive mutations are a subset of spontaneous types and their formation depends on the RecABC functions. Data presented here suggest that this phenomenon depends on transfer functions of the F factor. Fertility inhibition eliminates RecA-dependent adaptive reversion. Thus, "adaptive" revertants may form during replication from the transfer origin, whereas loci in the nonreplicating chromosome show little mutation.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 421-423 |
| Number of pages | 3 |
| Journal | Science |
| Volume | 268 |
| Issue number | 5209 |
| State | Published - 1995 |
| Externally published | Yes |
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Cite this
Evidence that F plasmid transfer replication underlies apparent adaptive mutation. / Galitski, Timothy; Roth, John R.
In: Science, Vol. 268, No. 5209, 1995, p. 421-423.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Evidence that F plasmid transfer replication underlies apparent adaptive mutation
AU - Galitski, Timothy
AU - Roth, John R.
PY - 1995
Y1 - 1995
N2 - An Escherichia coli K12 strain, FC40, has been used extensively in the analysis of adaptive mutability. This strain carries a revertible mutant lac allele on an F plasmid and accumulates Lac+ (lactose utilizing) revertants, but not unselected mutants, when placed on selective medium. These adaptive mutations are a subset of spontaneous types and their formation depends on the RecABC functions. Data presented here suggest that this phenomenon depends on transfer functions of the F factor. Fertility inhibition eliminates RecA-dependent adaptive reversion. Thus, "adaptive" revertants may form during replication from the transfer origin, whereas loci in the nonreplicating chromosome show little mutation.
AB - An Escherichia coli K12 strain, FC40, has been used extensively in the analysis of adaptive mutability. This strain carries a revertible mutant lac allele on an F plasmid and accumulates Lac+ (lactose utilizing) revertants, but not unselected mutants, when placed on selective medium. These adaptive mutations are a subset of spontaneous types and their formation depends on the RecABC functions. Data presented here suggest that this phenomenon depends on transfer functions of the F factor. Fertility inhibition eliminates RecA-dependent adaptive reversion. Thus, "adaptive" revertants may form during replication from the transfer origin, whereas loci in the nonreplicating chromosome show little mutation.
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UR - http://www.scopus.com/inward/citedby.url?scp=0029003985&partnerID=8YFLogxK
M3 - Article
VL - 268
SP - 421
EP - 423
JO - Science
JF - Science
SN - 0036-8075
IS - 5209
ER -