Evidence that F plasmid transfer replication underlies apparent adaptive mutation

Timothy Galitski, John R. Roth

Research output: Contribution to journalArticlepeer-review

116 Scopus citations


An Escherichia coli K12 strain, FC40, has been used extensively in the analysis of adaptive mutability. This strain carries a revertible mutant lac allele on an F plasmid and accumulates Lac+ (lactose utilizing) revertants, but not unselected mutants, when placed on selective medium. These adaptive mutations are a subset of spontaneous types and their formation depends on the RecABC functions. Data presented here suggest that this phenomenon depends on transfer functions of the F factor. Fertility inhibition eliminates RecA-dependent adaptive reversion. Thus, "adaptive" revertants may form during replication from the transfer origin, whereas loci in the nonreplicating chromosome show little mutation.

Original languageEnglish (US)
Pages (from-to)421-423
Number of pages3
Issue number5209
StatePublished - 1995
Externally publishedYes

ASJC Scopus subject areas

  • General


Dive into the research topics of 'Evidence that F plasmid transfer replication underlies apparent adaptive mutation'. Together they form a unique fingerprint.

Cite this