Evidence that different cation chloride cotransporters in retinal neurons allow opposite responses to GABA

N. Vardi, L. L. Zhang, John A Payne, P. Sterling

Research output: Contribution to journalArticle

135 Citations (Scopus)

Abstract

GABA gating an anion channel primarily permeable to chloride can hyperpolarize or depolarize, depending on whether the chloride equilibrium potential (E(Cl)) is negative or positive, respectively, to the resting membrane potential (E(rest)). If the transmembrane Cl- gradient is set by active transport, those neurons or neuronal regions that exhibit opposite responses to GABA should express different chloride transporters. To test this, we immunostained retina for the K-Cl cotransporter (KCC2) that normally extrudes chloride and for the Na-K-Cl cotransporter (NKCC) that normally accumulates chloride. KCC2 was expressed wherever E(Cl) is either known or predicted to be negative to E(rest) (ganglion cells, bipolar axons, and OFF bipolar dendrites), whereas NKCC was expressed wherever E(Cl) is either known or predicted to be positive to E(rest) (horizontal cells and ON bipolar dendrites). Thus, in the retina, the opposite effects of GABA on different cell types and on different cellula regions are probably primarily determined by the differential targeting of these two chloride transporters.

Original languageEnglish (US)
Pages (from-to)7657-7663
Number of pages7
JournalJournal of Neuroscience
Volume20
Issue number20
StatePublished - Oct 15 2000
Externally publishedYes

Fingerprint

Retinal Neurons
gamma-Aminobutyric Acid
Cations
Chlorides
Sodium-Potassium-Chloride Symporters
Dendrites
Retina
Active Biological Transport
Ganglia
Membrane Potentials
Anions
Axons
Neurons

Keywords

  • E(Cl)
  • GABA depolarization
  • Horizontal cells
  • KCC
  • NKCC
  • Receptive field
  • Retinal bipolar cells

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Evidence that different cation chloride cotransporters in retinal neurons allow opposite responses to GABA. / Vardi, N.; Zhang, L. L.; Payne, John A; Sterling, P.

In: Journal of Neuroscience, Vol. 20, No. 20, 15.10.2000, p. 7657-7663.

Research output: Contribution to journalArticle

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AU - Zhang, L. L.

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AB - GABA gating an anion channel primarily permeable to chloride can hyperpolarize or depolarize, depending on whether the chloride equilibrium potential (E(Cl)) is negative or positive, respectively, to the resting membrane potential (E(rest)). If the transmembrane Cl- gradient is set by active transport, those neurons or neuronal regions that exhibit opposite responses to GABA should express different chloride transporters. To test this, we immunostained retina for the K-Cl cotransporter (KCC2) that normally extrudes chloride and for the Na-K-Cl cotransporter (NKCC) that normally accumulates chloride. KCC2 was expressed wherever E(Cl) is either known or predicted to be negative to E(rest) (ganglion cells, bipolar axons, and OFF bipolar dendrites), whereas NKCC was expressed wherever E(Cl) is either known or predicted to be positive to E(rest) (horizontal cells and ON bipolar dendrites). Thus, in the retina, the opposite effects of GABA on different cell types and on different cellula regions are probably primarily determined by the differential targeting of these two chloride transporters.

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