Evidence that COG0325 proteins are involved in PLP homeostasis

Laurence Prunetti, Basma El Yacoubi, Cara R. Schiavon, Ericka Kirkpatrick, Lili Huang, Marc Bailly, Mona El Badawi-Sidhu, Katherine Harrison, Jesse F. Gregory, Oliver Fiehn, Andrew D. Hanson, Valérie De Crécy-Lagard

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Pyridoxal 5’-phosphate (PLP) is an essential cofactor for nearly 60 Escherichia coli enzymes but is a highly reactive molecule that is toxic in its free form. How PLP levels are regulated and how PLP is delivered to target enzymes are still open questions. The COG0325 protein family belongs to the fold-type III class of PLP enzymes and binds PLP but has no known biochemical activity although it occurs in all kingdoms of life. Various pleiotropic phenotypes of the E. coli COG0325 (yggS) mutant have been reported, some of which were reproduced and extended in this study. Comparative genomic, genetic and metabolic analyses suggest that these phenotypes reflect an imbalance in PLP homeostasis. The E. coli yggS mutant accumulates the PLP precursor pyridoxine 5’-phosphate (PNP) and is sensitive to an excess of pyridoxine but not of pyridoxal. The pyridoxine toxicity phenotype is complemented by the expression of eukaryotic yggS orthologs. It is also suppressed by the presence of amino acids, specifically isoleucine, threonine and leucine, suggesting the PLP-dependent enzyme transaminase B (IlvE) is affected. These genetic results lay a foundation for future biochemical studies of the role of COG0325 proteins in PLP homeostasis.

Original languageEnglish (US)
Pages (from-to)694-706
Number of pages13
JournalMicrobiology (United Kingdom)
Volume162
Issue number4
DOIs
StatePublished - Apr 1 2016

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Pyridoxal Phosphate
Homeostasis
Proteins
Pyridoxine
Enzymes
Escherichia coli
Phenotype
Pyridoxal
Isoleucine
Poisons
Threonine
Leucine
Amino Acids

ASJC Scopus subject areas

  • Microbiology

Cite this

Prunetti, L., El Yacoubi, B., Schiavon, C. R., Kirkpatrick, E., Huang, L., Bailly, M., ... De Crécy-Lagard, V. (2016). Evidence that COG0325 proteins are involved in PLP homeostasis. Microbiology (United Kingdom), 162(4), 694-706. https://doi.org/10.1099/mic.0.000255

Evidence that COG0325 proteins are involved in PLP homeostasis. / Prunetti, Laurence; El Yacoubi, Basma; Schiavon, Cara R.; Kirkpatrick, Ericka; Huang, Lili; Bailly, Marc; El Badawi-Sidhu, Mona; Harrison, Katherine; Gregory, Jesse F.; Fiehn, Oliver; Hanson, Andrew D.; De Crécy-Lagard, Valérie.

In: Microbiology (United Kingdom), Vol. 162, No. 4, 01.04.2016, p. 694-706.

Research output: Contribution to journalArticle

Prunetti, L, El Yacoubi, B, Schiavon, CR, Kirkpatrick, E, Huang, L, Bailly, M, El Badawi-Sidhu, M, Harrison, K, Gregory, JF, Fiehn, O, Hanson, AD & De Crécy-Lagard, V 2016, 'Evidence that COG0325 proteins are involved in PLP homeostasis', Microbiology (United Kingdom), vol. 162, no. 4, pp. 694-706. https://doi.org/10.1099/mic.0.000255
Prunetti L, El Yacoubi B, Schiavon CR, Kirkpatrick E, Huang L, Bailly M et al. Evidence that COG0325 proteins are involved in PLP homeostasis. Microbiology (United Kingdom). 2016 Apr 1;162(4):694-706. https://doi.org/10.1099/mic.0.000255
Prunetti, Laurence ; El Yacoubi, Basma ; Schiavon, Cara R. ; Kirkpatrick, Ericka ; Huang, Lili ; Bailly, Marc ; El Badawi-Sidhu, Mona ; Harrison, Katherine ; Gregory, Jesse F. ; Fiehn, Oliver ; Hanson, Andrew D. ; De Crécy-Lagard, Valérie. / Evidence that COG0325 proteins are involved in PLP homeostasis. In: Microbiology (United Kingdom). 2016 ; Vol. 162, No. 4. pp. 694-706.
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