Evidence that a B12-adenosyl transferase is encoded within the ethanolamine operon of Salmonella enterica

David E. Sheppard, Joseph T. Penrod, Thomas Bobik, Eric Kofoid, John R. Roth

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

Adenosylcobalamin (Ado-B12) is both the cofactor and inducer of ethanolamine ammonia lyase (EA-lyase), a catabolic enzyme for ethanolamine. De novo synthesis of Ado-B12 by Salmonella enterica occurs only under anaerobic conditions. Therefore, aerobic growth on ethanolamine requires import of Ado-B12 or a precursor (CN-B12 or OH-B12) that can be adenosylated internally. Several known enzymes adenosylate corrinoids. The CobA enzyme transfers adenosine from ATP to a biosynthetic intermediate in de novo B12 synthesis and to imported CN-B 12, OH-B12, or Cbi (a B12 precursor). The PduO adenosyl transferase is encoded in an operon (pdu) for cobalamin-dependent propanediol degradation and is induced by propanediol. Evidence is presented here that a third transferase (EutT) is encoded within the operon for ethanolamine utilization (eut). Surprisingly, these three transferases share no apparent sequence similarity. CobA produces sufficient Ado-B12 to initiate eut operon induction and to serve as a cofactor for EA-lyase when B12 levels are high. Once the eut operon is induced, the EutT transferase supplies more Ado-B12 during the period of high demand. Another protein encoded in the operon (EutA) protects EA-lyase from inhibition by CN-B12 but does so without adenosylation of this corrinoid.

Original languageEnglish (US)
Pages (from-to)7635-7644
Number of pages10
JournalJournal of Bacteriology
Volume186
Issue number22
DOIs
StatePublished - Nov 2004

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Ethanolamine
Salmonella enterica
Ethanolamine Ammonia-Lyase
Operon
Transferases
Corrinoids
Propylene Glycols
Enzymes
Vitamin B 12
Adenosine
Adenosine Triphosphate
Growth
Proteins

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Immunology

Cite this

Evidence that a B12-adenosyl transferase is encoded within the ethanolamine operon of Salmonella enterica. / Sheppard, David E.; Penrod, Joseph T.; Bobik, Thomas; Kofoid, Eric; Roth, John R.

In: Journal of Bacteriology, Vol. 186, No. 22, 11.2004, p. 7635-7644.

Research output: Contribution to journalArticle

Sheppard, David E. ; Penrod, Joseph T. ; Bobik, Thomas ; Kofoid, Eric ; Roth, John R. / Evidence that a B12-adenosyl transferase is encoded within the ethanolamine operon of Salmonella enterica. In: Journal of Bacteriology. 2004 ; Vol. 186, No. 22. pp. 7635-7644.
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