Evidence of simian retrovirus type D by polymerase chain reaction

Christian Z R Hwa, Sheung Pun Tsai, Joann L. Yee, Koen K. Van Rompay, Jeffrey A Roberts

Research output: Contribution to journalArticle

Abstract

Background: Over the past few years, there have been reports of finding Simian retrovirus type D (SRV) in macaque colonies where some animals were characterized as antibody positive but virus negative raising questions about how SRV was transmitted or whether there is a variant strain detected by antibody but not polymerase chain reaction (PCR) in current use. Methods: We developed a three-round nested PCR assay using degenerate primers targeting the pol gene to detect for SRV serotypes 1-5 and applied this newly validated PCR assay to test macaque DNA samples collected in China from 2010 to 2015. Results: Using the nested PCR assay validated in this study, we found 0.15% of the samples archived on FTA® cards were positive. Conclusions: The source of SRV infection identified within domestic colonies might have originated from imported macaques. The multiplex nested PCR assay developed here may supplement the current assays for SRV.

Original languageEnglish (US)
JournalJournal of Medical Primatology
DOIs
StateAccepted/In press - 2017

Fingerprint

Simian Retroviruses
Betaretrovirus
polymerase chain reaction
Macaca
Polymerase Chain Reaction
assays
Mason-Pfizer monkey virus
pol Genes
antibodies
Antibodies
Multiplex Polymerase Chain Reaction
gene targeting
China
serotypes
Viruses
sampling
viruses
DNA
Infection
infection

Keywords

  • Cynomolgus macaque
  • FTA card
  • Nested polymerase chain reaction
  • Rhesus macaque
  • Simian retrovirus

ASJC Scopus subject areas

  • Animal Science and Zoology
  • veterinary(all)

Cite this

Evidence of simian retrovirus type D by polymerase chain reaction. / Hwa, Christian Z R; Tsai, Sheung Pun; Yee, Joann L.; Van Rompay, Koen K.; Roberts, Jeffrey A.

In: Journal of Medical Primatology, 2017.

Research output: Contribution to journalArticle

Hwa, Christian Z R ; Tsai, Sheung Pun ; Yee, Joann L. ; Van Rompay, Koen K. ; Roberts, Jeffrey A. / Evidence of simian retrovirus type D by polymerase chain reaction. In: Journal of Medical Primatology. 2017.
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