Evidence of Muscovy duck parvovirus in Muscovy ducklings in California

P. R. Woolcock, V. Jestin, H L Shivaprasad, F. Zwingelstein, C. Arnauld, M. D. McFarland, J. C. Pedersen, D. A. Senne

Research output: Contribution to journalArticle

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Abstract

Muscovy duck parvovirus (MDPV) has been demonstrated in tissue samples from one-to four-week-old commercially reared Muscovy ducks that were weak, unable to walk and had a high mortality rate. On postmortem examination, the thigh and leg muscles, and the myocardium were found to be pale, and there was a fibrinous exudate on the capsule of the liver, and ascites. The parvovirus was isolated in embryonated Muscovy duck eggs and visualised by negative stain electron microscopy, detected by polymerase chain reaction (PCR) directly from the tissues, and antibodies to it were detected by immunoelectron microscopy, ELISA and immunofluorescence. In addition, the PCR products obtained that represented 1625 bp (74 per cent) of the capsid VP1 gene, including a hypervariable region between Derzsy's disease virus or goose parvovirus and MDPV, were sequenced and shown to be 100 per cent homologous with the MDPV 89384 reference strain, but only 82-3 per cent homologous with Derzsy's disease virus.

Original languageEnglish (US)
Pages (from-to)68-74
Number of pages7
JournalVeterinary Record
Volume146
Issue number3
StatePublished - Jan 15 2000

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Muscovy
Parvovirus
ducklings
Ducks
Cairina moschata
Virus Diseases
polymerase chain reaction
duck eggs
viruses
Protoparvovirus
capsid
ascites
Geese
thighs
myocardium
Polymerase Chain Reaction
Immunoelectron Microscopy
fluorescent antibody technique
Capsid
Exudates and Transudates

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Woolcock, P. R., Jestin, V., Shivaprasad, H. L., Zwingelstein, F., Arnauld, C., McFarland, M. D., ... Senne, D. A. (2000). Evidence of Muscovy duck parvovirus in Muscovy ducklings in California. Veterinary Record, 146(3), 68-74.

Evidence of Muscovy duck parvovirus in Muscovy ducklings in California. / Woolcock, P. R.; Jestin, V.; Shivaprasad, H L; Zwingelstein, F.; Arnauld, C.; McFarland, M. D.; Pedersen, J. C.; Senne, D. A.

In: Veterinary Record, Vol. 146, No. 3, 15.01.2000, p. 68-74.

Research output: Contribution to journalArticle

Woolcock, PR, Jestin, V, Shivaprasad, HL, Zwingelstein, F, Arnauld, C, McFarland, MD, Pedersen, JC & Senne, DA 2000, 'Evidence of Muscovy duck parvovirus in Muscovy ducklings in California', Veterinary Record, vol. 146, no. 3, pp. 68-74.
Woolcock PR, Jestin V, Shivaprasad HL, Zwingelstein F, Arnauld C, McFarland MD et al. Evidence of Muscovy duck parvovirus in Muscovy ducklings in California. Veterinary Record. 2000 Jan 15;146(3):68-74.
Woolcock, P. R. ; Jestin, V. ; Shivaprasad, H L ; Zwingelstein, F. ; Arnauld, C. ; McFarland, M. D. ; Pedersen, J. C. ; Senne, D. A. / Evidence of Muscovy duck parvovirus in Muscovy ducklings in California. In: Veterinary Record. 2000 ; Vol. 146, No. 3. pp. 68-74.
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