Abstract
Background: In men with prostate cancer, the gland usually contains two or more widely separate tumors. A critical issue of prostatic carcinogenesis is whether these multiple tumors are independent in origin. Molecular analysis of microsatellite (i.e., highly repeated, short nucleotide sequences) alterations in the DNA from separate tumors in the same prostate can be used to determine whether or not these separate tumors arise independently. Methods: Four microsatellite polymorphic markers (D8S133, D8S136, and D8S137, for a putative tumor suppressor gene on chromosome 8p, and D17S855, for the BRCA1 gene on chromosome 17q) were used to examine the pattern of allelic loss in prostate cancer from 19 patients who had two or more distantly separate tumors (i.e., located on contralateral sides or separated by at least half the anterior-posterior diameter of the prostate). Forty distantly separate tumors were microdissected, DNA samples were prepared from formalin-fixed, paraffin-embedded wholemount prostate tissue section, and the overall frequencies of loss of heterozygosity at the four loci were determined. Results: The pattern of allelic loss was compatible with independent tumor origin in 15 of 18 informative cases. A random discordant pattern of allelic deletion was observed in distantly separate tumors, whereas the same allele was consistently lost in cells from different regions of the same tumor. For three patients, the results were compatible with either intraglandular dissemination or independent origin of prostate cancer. Conclusions: Our data suggest that multiple tumors in some patients with prostate cancer have independent origin.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 233-237 |
| Number of pages | 5 |
| Journal | Journal of the National Cancer Institute |
| Volume | 90 |
| Issue number | 3 |
| State | Published - Feb 4 1998 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Cancer Research
- Oncology
Cite this
Evidence of independent origin of multiple tumors from patients with prostate cancer. / Cheng, Liang; Song, Sang Yong; Pretlow, Thomas G.; Abdul-Karim, Fadi W.; Kung, Hsing-Jien; Dawson, Deborah V.; Park, Won Sang; Moon, Young Wan; Tsai, Min Lung; Linehan, W. Marston; Emmert-Buck, Michael R.; Liotta, Lance A.; Zhuang, Zhengping.
In: Journal of the National Cancer Institute, Vol. 90, No. 3, 04.02.1998, p. 233-237.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Evidence of independent origin of multiple tumors from patients with prostate cancer
AU - Cheng, Liang
AU - Song, Sang Yong
AU - Pretlow, Thomas G.
AU - Abdul-Karim, Fadi W.
AU - Kung, Hsing-Jien
AU - Dawson, Deborah V.
AU - Park, Won Sang
AU - Moon, Young Wan
AU - Tsai, Min Lung
AU - Linehan, W. Marston
AU - Emmert-Buck, Michael R.
AU - Liotta, Lance A.
AU - Zhuang, Zhengping
PY - 1998/2/4
Y1 - 1998/2/4
N2 - Background: In men with prostate cancer, the gland usually contains two or more widely separate tumors. A critical issue of prostatic carcinogenesis is whether these multiple tumors are independent in origin. Molecular analysis of microsatellite (i.e., highly repeated, short nucleotide sequences) alterations in the DNA from separate tumors in the same prostate can be used to determine whether or not these separate tumors arise independently. Methods: Four microsatellite polymorphic markers (D8S133, D8S136, and D8S137, for a putative tumor suppressor gene on chromosome 8p, and D17S855, for the BRCA1 gene on chromosome 17q) were used to examine the pattern of allelic loss in prostate cancer from 19 patients who had two or more distantly separate tumors (i.e., located on contralateral sides or separated by at least half the anterior-posterior diameter of the prostate). Forty distantly separate tumors were microdissected, DNA samples were prepared from formalin-fixed, paraffin-embedded wholemount prostate tissue section, and the overall frequencies of loss of heterozygosity at the four loci were determined. Results: The pattern of allelic loss was compatible with independent tumor origin in 15 of 18 informative cases. A random discordant pattern of allelic deletion was observed in distantly separate tumors, whereas the same allele was consistently lost in cells from different regions of the same tumor. For three patients, the results were compatible with either intraglandular dissemination or independent origin of prostate cancer. Conclusions: Our data suggest that multiple tumors in some patients with prostate cancer have independent origin.
AB - Background: In men with prostate cancer, the gland usually contains two or more widely separate tumors. A critical issue of prostatic carcinogenesis is whether these multiple tumors are independent in origin. Molecular analysis of microsatellite (i.e., highly repeated, short nucleotide sequences) alterations in the DNA from separate tumors in the same prostate can be used to determine whether or not these separate tumors arise independently. Methods: Four microsatellite polymorphic markers (D8S133, D8S136, and D8S137, for a putative tumor suppressor gene on chromosome 8p, and D17S855, for the BRCA1 gene on chromosome 17q) were used to examine the pattern of allelic loss in prostate cancer from 19 patients who had two or more distantly separate tumors (i.e., located on contralateral sides or separated by at least half the anterior-posterior diameter of the prostate). Forty distantly separate tumors were microdissected, DNA samples were prepared from formalin-fixed, paraffin-embedded wholemount prostate tissue section, and the overall frequencies of loss of heterozygosity at the four loci were determined. Results: The pattern of allelic loss was compatible with independent tumor origin in 15 of 18 informative cases. A random discordant pattern of allelic deletion was observed in distantly separate tumors, whereas the same allele was consistently lost in cells from different regions of the same tumor. For three patients, the results were compatible with either intraglandular dissemination or independent origin of prostate cancer. Conclusions: Our data suggest that multiple tumors in some patients with prostate cancer have independent origin.
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UR - http://www.scopus.com/inward/citedby.url?scp=0032481322&partnerID=8YFLogxK
M3 - Article
C2 - 9462681
AN - SCOPUS:0032481322
VL - 90
SP - 233
EP - 237
JO - Journal of the National Cancer Institute
JF - Journal of the National Cancer Institute
SN - 0027-8874
IS - 3
ER -