Evidence of genome segment 5 reassortment in bluetongue virus field isolates.

C. C. de Mattos, C. A. de Mattos, Bennie Osburn, M. Ianconescu, R. Kaufman

Research output: Contribution to journalArticlepeer-review

14 Scopus citations

Abstract

A recombinant cDNA probe from genome segment 5 obtained from a virulent US bluetongue virus strain (BTV-11 strain UC8) was hybridized to US and Israeli BTV prototypes and field isolates. The cloned genetic probe hybridized with US BTV prototype 10, but not with US prototypes 2, 11, 13, and 17; with the avirulent BTV-11 strain UC2; and with the Israeli prototype 10. When the probe was hybridized to field isolates from the US serotypes, it hybridized to 12 of 14 BTV-10 isolates and 4 of 17 BTV-11 samples, but not to the BTV-13 and BTV-17 samples tested. Hybridization was not observed with the Israeli field isolates studied. Results indicate that a reassortant event occurred between a strain of US BTV-10 and US BTV-11 that originated the BTV-11 strain UC8.

Original languageEnglish (US)
Pages (from-to)1794-1798
Number of pages5
JournalAmerican Journal of Veterinary Research
Volume52
Issue number11
StatePublished - Nov 1 1991

ASJC Scopus subject areas

  • veterinary(all)

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