Evidence for symmetric chromosomal inversions around the replication origin in bacteria.

Jonathan A Eisen, J. F. Heidelberg, O. White, S. L. Salzberg

Research output: Contribution to journalArticle

213 Citations (Scopus)

Abstract

BACKGROUND: Whole-genome comparisons can provide great insight into many aspects of biology. Until recently, however, comparisons were mainly possible only between distantly related species. Complete genome sequences are now becoming available from multiple sets of closely related strains or species. RESULTS: By comparing the recently completed genome sequences of Vibrio cholerae, Streptococcus pneumoniae and Mycobacterium tuberculosis to those of closely related species - Escherichia coli, Streptococcus pyogenes and Mycobacterium leprae, respectively - we have identified an unusual and previously unobserved feature of bacterial genome structure. Scatterplots of the conserved sequences (both DNA and protein) between each pair of species produce a distinct X-shaped pattern, which we call an X-alignment. The key feature of these alignments is that they have symmetry around the replication origin and terminus; that is, the distance of a particular conserved feature (DNA or protein) from the replication origin (or terminus) is conserved between closely related pairs of species. Statistically significant X-alignments are also found within some genomes, indicating that there is symmetry about the replication origin for paralogous features as well. CONCLUSIONS: The most likely mechanism of generation of X-alignments involves large chromosomal inversions that reverse the genomic sequence symmetrically around the origin of replication. The finding of these X-alignments between many pairs of species suggests that chromosomal inversions around the origin are a common feature of bacterial genome evolution.

Original languageEnglish (US)
JournalGenome Biology
Volume1
Issue number6
StatePublished - 2000
Externally publishedYes

Fingerprint

replication origin
Replication Origin
genome
Genome
Bacteria
Bacterial Genomes
bacterium
bacteria
Bacterial Structures
Mycobacterium leprae
Vibrio cholerae
symmetry
Conserved Sequence
Streptococcus pyogenes
DNA
Streptococcus pneumoniae
Mycobacterium tuberculosis
Proteins
cholera
protein

ASJC Scopus subject areas

  • Genetics

Cite this

Evidence for symmetric chromosomal inversions around the replication origin in bacteria. / Eisen, Jonathan A; Heidelberg, J. F.; White, O.; Salzberg, S. L.

In: Genome Biology, Vol. 1, No. 6, 2000.

Research output: Contribution to journalArticle

Eisen, Jonathan A ; Heidelberg, J. F. ; White, O. ; Salzberg, S. L. / Evidence for symmetric chromosomal inversions around the replication origin in bacteria. In: Genome Biology. 2000 ; Vol. 1, No. 6.
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