Evidence for leaflet-dependent redistribution of charged molecules in fluid supported phospholipid bilayers

Andrew P. Shreve, Michael C. Howland, Annapoorna R. Sapuri-Butti, Toby W. Allen, Atul N. Parikh

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

The asymmetric distribution of charged molecules between the leaflets of solid-substrate-supported phospholipid bilayers is studied using imaging ellipsometry, fluorescence microscopy, and numerical solutions of the Poisson-Boltzmann equation. Experiments are facilitated by the use of patterned substrates that allow for side-by-side comparison of lipid monolayers and supported bilayers. On silica surfaces, negatively charged lipid components are shown to be enriched in the outer leaflet of a supported bilayer system at modest salt concentrations. The approaches developed provide a general means for determining asymmetries of charged components in supported lipid bilayers.

Original languageEnglish (US)
Pages (from-to)13250-13253
Number of pages4
JournalLangmuir
Volume24
Issue number23
DOIs
StatePublished - Dec 2 2008

Fingerprint

Phospholipids
Lipids
lipids
Molecules
Lipid bilayers
Fluids
Boltzmann equation
fluids
Fluorescence microscopy
Ellipsometry
Substrates
Silicon Dioxide
molecules
Monolayers
Salts
Silica
Imaging techniques
ellipsometry
asymmetry
silicon dioxide

ASJC Scopus subject areas

  • Electrochemistry
  • Condensed Matter Physics
  • Surfaces and Interfaces
  • Materials Science(all)
  • Spectroscopy

Cite this

Shreve, A. P., Howland, M. C., Sapuri-Butti, A. R., Allen, T. W., & Parikh, A. N. (2008). Evidence for leaflet-dependent redistribution of charged molecules in fluid supported phospholipid bilayers. Langmuir, 24(23), 13250-13253. https://doi.org/10.1021/la802909c

Evidence for leaflet-dependent redistribution of charged molecules in fluid supported phospholipid bilayers. / Shreve, Andrew P.; Howland, Michael C.; Sapuri-Butti, Annapoorna R.; Allen, Toby W.; Parikh, Atul N.

In: Langmuir, Vol. 24, No. 23, 02.12.2008, p. 13250-13253.

Research output: Contribution to journalArticle

Shreve, AP, Howland, MC, Sapuri-Butti, AR, Allen, TW & Parikh, AN 2008, 'Evidence for leaflet-dependent redistribution of charged molecules in fluid supported phospholipid bilayers', Langmuir, vol. 24, no. 23, pp. 13250-13253. https://doi.org/10.1021/la802909c
Shreve, Andrew P. ; Howland, Michael C. ; Sapuri-Butti, Annapoorna R. ; Allen, Toby W. ; Parikh, Atul N. / Evidence for leaflet-dependent redistribution of charged molecules in fluid supported phospholipid bilayers. In: Langmuir. 2008 ; Vol. 24, No. 23. pp. 13250-13253.
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