Evidence for leaflet-dependent redistribution of charged molecules in fluid supported phospholipid bilayers

Andrew P. Shreve, Michael C. Howland, Annapoorna R. Sapuri-Butti, Toby W. Allen, Atul N. Parikh

Research output: Contribution to journalArticle

33 Scopus citations

Abstract

The asymmetric distribution of charged molecules between the leaflets of solid-substrate-supported phospholipid bilayers is studied using imaging ellipsometry, fluorescence microscopy, and numerical solutions of the Poisson-Boltzmann equation. Experiments are facilitated by the use of patterned substrates that allow for side-by-side comparison of lipid monolayers and supported bilayers. On silica surfaces, negatively charged lipid components are shown to be enriched in the outer leaflet of a supported bilayer system at modest salt concentrations. The approaches developed provide a general means for determining asymmetries of charged components in supported lipid bilayers.

Original languageEnglish (US)
Pages (from-to)13250-13253
Number of pages4
JournalLangmuir
Volume24
Issue number23
DOIs
StatePublished - Dec 2 2008

ASJC Scopus subject areas

  • Electrochemistry
  • Condensed Matter Physics
  • Surfaces and Interfaces
  • Materials Science(all)
  • Spectroscopy

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    Shreve, A. P., Howland, M. C., Sapuri-Butti, A. R., Allen, T. W., & Parikh, A. N. (2008). Evidence for leaflet-dependent redistribution of charged molecules in fluid supported phospholipid bilayers. Langmuir, 24(23), 13250-13253. https://doi.org/10.1021/la802909c